Korean J Anesthesiol.  2004 Aug;47(2):253-259. 10.4097/kjae.2004.47.2.253.

Effect of Etomidate on the Expressions of c-JUN and Heat Shock Protein 70 Induced by Kainic Acid in Rats

• Lee JH
• Lee YM
• Chang BJ
• Park SJ
• Lee JY
• Kim JU
• Ku SW

• KMID: 2237538
• DOI: http://doi.org/10.4097/kjae.2004.47.2.253

Abstract

BACKGROUND: The present investigation was undertaken to evaluate the neuroprotective effect of etomidate against kainic acid (KA) induced neurotoxicity in rats by Western immunoblotting for heat shock protein 70 (HSP-70), c-JUN, and by acid-fuchsin histochemistry.
METHODS
Etomidate (20 mg/kg, i.p.) was administered in sequence, first being just one hour after KA (10 mg/kg, i.p.) injection, then three times one hour intervals. Neuronal damage in hippocampus was evaluated by using acid-fuchsin stain to detect cell death and HSP-70 and c-JUN induction as an index of cell injury at 3, 6, 24 and 48 h after the administration of KA.
RESULTS
Acid fuchsin positive neurons were increased in the CA1 and CA3 regions of the hippocampus after KA injection, but were significantly decreased by etomidate injection (P <0.01). Etomidate administration also significantly suppressed the KA-induced induction of c-JUN and HSP-70 in both regions of the rat hippocampus.
CONCLUSIONS
These results suggest that etomidate has a protective effect on hippocampal neurons against KA-induced neurotoxicity.

Keyword

c-JUN; etomidate; hippocampus; heat shock protein 70; kainic acid; neurotoxicity

MeSH Terms

Animals
Blotting, Western
Cell Death
Etomidate*
Heat-Shock Proteins*
Hippocampus
Hot Temperature*
HSP70 Heat-Shock Proteins*
Kainic Acid*
Neurons
Neuroprotective Agents
Rats*
Rosaniline Dyes
Etomidate
HSP70 Heat-Shock Proteins
Heat-Shock Proteins
Kainic Acid
Neuroprotective Agents
Rosaniline Dyes