Toxicol Res.  2012 Sep;28(3):159-164.

External Application of Fermented Olive Flounder (Paralichthys olivaceus) Oil Alleviates Inflammatory Responses in 2,4-Dinitrochlorobenzene-induced Atopic Dermatitis Mouse Model

• Han SC
• Kang GJ
• Ko YJ
• Kang HK
• Moon SW
• Ann YS
• Yoo ES

Abstract

Allergic skin inflammation such as atopic dermatitis (AD) is characterized by edema and infiltration with various inflammatory cells such as mast cells, basophils, eosinophils and T cells. Thymic stromal lymphopoietin (TSLP) is produced mainly by epidermal keratinocytes, as well as dermal fibroblasts and mast cells in the skin lesions of AD. Omega-3 polyunsaturated fatty acids in fish oil can reduce inflammation in allergic patients. Fermentation has a tremendous capacity to transform chemical structures. The anti-inflammatory effects of fish oil have been described in many diseases, but the beneficial effects by which fermented olive flounder oil (FOF) modulates the allergic response is poorly understood. In this study, we produced FOF and tested its ability to suppress the various allergic inflammatory responses. The ability of FOF to modulate the immune system was investigated using a mouse model of AD. The FOF-treated group showed significantly decreased immunoglobulin E (IgE) and histamine in serum. Also, the increased TSLP expression was significantly inhibited in the FOF group; the FOF-treated group was not appreciably different from the hydrocort cream treatment group. In addition, FOF treatment resulted in a smaller spleen size with reduced the thickness and length compared to the induction group. Splenocytes from mice treated with FOF produced significantly less IFN-gamma, IL-4, T-box transcription factor (T-bet) and GATA binding protein 3 (GATA3) expression compared with the induction group. These results suggest that FOF may be effective in treating the allergic symptoms of AD. 5.

Keyword

Atopic dermatitis; Fermented olive flounder oil; Thymic stromal lymphopoietin; T-box transcription factor; GATA binding protein 3

MeSH Terms

Animals
Basophils
Carrier Proteins
Cytokines
Dermatitis, Atopic
Edema
Eosinophils
Fatty Acids, Unsaturated
Fermentation
Fibroblasts
Flounder
Histamine
Humans
Immune System
Immunoglobulin E
Immunoglobulins
Inflammation
Interleukin-4
Keratinocytes
Mast Cells
Mice
Olea
Skin
Spleen
T-Lymphocytes
Transcription Factors
Carrier Proteins
Cytokines
Fatty Acids, Unsaturated
Histamine
Immunoglobulin E
Immunoglobulins
Interleukin-4
Transcription Factors