Nutr Res Pract.  2016 Apr;10(2):154-160. 10.4162/nrp.2016.10.2.154.

Effects of Cyclo-His-Pro-enriched yeast hydrolysate on blood glucose levels and lipid metabolism in obese diabetic ob/ob mice

• Jung EY
• Hong YH
• Park C
• Suh HJ

• KMID: 2313912
• DOI: http://doi.org/10.4162/nrp.2016.10.2.154

Abstract

BACKGROUND/OBJECTIVES
We examined the hypoglycemic and anti-hyperlipidemic effect of yeast hydrolysate (YH) enriched with Cyclo-His-Pro (CHP) in the C57BL/6J ob/ob mouse model.
MATERIALS/METHODS
Mice were separated into 4 groups (8 mice/group) on the basis of blood glucose and body weight: WT control, lean mice given vehicle; ob/ob control, ob/ob mice given vehicle; YH-1, ob/ob mice given 0.5 g/kg of YH; YH-2, ob/ob mice given 1 g/kg of YH. YH in saline or vehicle was administered orally in the same volume every day for 3 weeks.
RESULTS
Mice treated with YH (0.5 and 1 g/kg) for 3 weeks displayed a significant reduction in overall body weight gain and perirenal and epididymal adipose tissue weight compared to the ob/ob control group. Additionally, high-density lipoprotein (HDL) cholesterol, glucose, and atherogenic indexes were significantly decreased in the blood of YH-1 and YH-2 groups compared to the ob/ob control. In ob/ob mice, YH administration significantly improved glucose tolerance and blood insulin levels. These data indicate that YH treatment produces potent hypoglycemic and anti-hyperlipidemic effects by controlling body weight, fat mass, blood lipid, insulin levels, and glucose tolerance.
CONCLUSION
YH could potentially be used as a treatment option for diabetes and hyperlipidemia. The CHP-enriched YH may be a promising strategy in the development of hypoglycemic peptide nutraceuticals.

Keyword

Yeast hydrolysate; blood glucose; diabetes mellitus; cyclo-his-pro

MeSH Terms

Adipose Tissue
Animals
Blood Glucose*
Body Weight
Cholesterol
Diabetes Mellitus
Dietary Supplements
Glucose
Hyperlipidemias
Insulin
Lipid Metabolism*
Lipoproteins
Mice*
Yeasts*
Blood Glucose
Cholesterol
Glucose
Insulin
Lipoproteins

Figure

• 

  Fig. 1 Effects of yeast hydrolysate (YH) on oral glucose tolerance test (A, OGTT) and blood insulin levels (B) in ob/ob mice. Values are the mean ± SEM for 8 mice. Values with different superscript letters are significantly different at P < 0.05 by Duncan's multiple range test. WT control, lean mice given vehicle; ob/ob control, ob/ob mice given vehicle; YH-1, ob/ob mice given 0.5 g/kg of YH; YH-2, ob/ob mice given 1 g/kg of YH. Incremental areas from the baseline under the response curves (AUC) were calculated using the trapezoidal rule.

• 

  Fig. 2 Effects of yeast hydrolysate (YH) on the immunoreactivities of insulin (A) and glucagon (B) in pancreatic islets of ob/ob mice. Values are the mean ± SEM for 8 mice. Values with different superscript letters are significantly different at P < 0.05 by Duncan's multiple range test. WT control, lean mice given vehicle; ob/ob control, ob/ob mice given vehicle; YH-1, ob/ob mice given 0.5 g/kg of YH; YH-2, ob/ob mice given 1 g/kg of YH.

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