Nutr Res Pract.  2014 Apr;8(2):158-164.

Peanut sprouts extract (Arachis hypogaea L.) has anti-obesity effects by controlling the protein expressions of PPARgamma and adiponectin of adipose tissue in rats fed high-fat diet

• Kang NE
• Ha AW
• Woo HW
• Kim WK

Abstract

BACKGROUD/OBEJECTIVES: This study aims to find out the effects of peanut sprout extracts on weight controls and protein expressions of transcription factors related to adipocyte differentiation and adipocytokine in rats under high-fat diets.
MATERIALS/METHODS
Four week-old Sparague-Dawley (SD) were assigned to 4 groups; normal-fat (NF) diets (7% fat diet), high-fat (HF) diets (20% fat diet), high fat diets with low peanut sprout extract (HF + PSEL) diet (20% fat and 0.025% peanut sprout extract), and high fat diets with high peanut sprout extract (HF + PSEH) diet (20% fat and 0.05% peanut sprout extract). Body weight changes, lipid profiles in adipose tissue, and the mRNA protein expressions, such as peroxisome proliferator-activated receptor gamma (PPARgamma), CCAAT element binding protein alpha (C/EBP alpha), leptin, and adiponectin, were determined.
RESULTS
After 9 weeks of feeding, the HF + PSEH group had significantly less weight gains than the HF group (P < 0.05). However, the total dietary intakes or food efficiency ratios among groups were not significantly different. The weight of epididymal fat in HF + PSEH group, 3.61 +/- 0.5 g, or HF + PSEL group, 3.80 +/- 0.7 g, was significantly lower than the HF group, 4.39 +/- 0.4g, (P < 0.05). Total lipids and total cholesterol in adipose tissue were significantly decreased in HF + PSEH group compared to those in the HF group, respectively (P < 0.05). PSEH supplementation caused AST and ALT levels to decrease when it compared to HF group, but it was not statistically significant. The protein expression of PPARgamma in HF + PSEH group was significantly lower than the HF group (P < 0.05). Comparing with the HF group, the protein expression of adiponectin in HF + PSEH group was significantly increased (P < 0.05). The protein expressions of C/EBP alpha and leptin in HF + PSEH group were lower than the HF group, but it was not statistical significant.
CONCLUSIONS
In conclusion, peanut sprout extract has anti-obesity effect by lowering the expressions of PPARgamma which regulates the expression of adiponectin.

Keyword

Peanut sprout extract; obesity; PPARgamma; adiponectin; high fat diet

MeSH Terms

Adipocytes
Adiponectin*
Adipose Tissue*
Animals
Body Weight Changes
Carrier Proteins
Cholesterol
Diet
Diet, High-Fat*
Leptin
Obesity
PPAR gamma*
Rats*
RNA, Messenger
Transcription Factors
Weight Gain
Adiponectin
Carrier Proteins
Cholesterol
Leptin
PPAR gamma
RNA, Messenger
Transcription Factors

Figure

• 

  Fig. 1 Effects of peanut sprout extracts on PPARγ expression in adipose tissues of rats. Frozen adipose tissue was homogenized in lysis buffer. Protein concentration was determined by using a Bio-Rad method. Equal amounts of proteins (30 µg) were resolved by SDS-PAGE, transferred to the membranes and probed with PPARγ. Above photographs of chemiliuminiscent detection of the western blots, are shown and under the graph of quantitative analysis which were representatives of three independent experiments. Each bar represents the mean ± SE (n = 10). Comparison among different concentrations of peanut sprout extracts that yielded significant differences (P < 0.05) are indicated by the different letters above each bar.

• 

  Fig. 2 Effect of peanut sprout extracts on C/EBPα expression in adipose tissue of rats. Frozen adipose tissue was homogenized in lysis buffer. Protein concentration was determined by using a Bio-Rad method. Equal amounts of proteins (30 µg) were resolved by SDS-PAGE, transferred to the membranes and probed with C/EBPα. Above photographs of chemiliuminiscent detection of the western blots, are shown and under the graph of quantitative analysis which were representatives of three independent experiments. Each bar represents the mean ± SE

• 

  Fig. 3 Effect of peanut sprout extracts on adiponectin expression in adipose tissue of rats. Frozen adipose tissue was homogenized in lysis buffer. Protein concentration was determined by using a Bio-Rad method. Equal amounts of proteins (30 µg) were resolved by SDS-PAGE, transferred to the membranes and probed with adiponectin. Above photographs of chemiliuminiscent detection of the western blots, are shown and under the graph of quantitative analysis which were representatives of three independent experiments. Each bar represents the mean ± SE Comparison among different concentrations of peanut sprout extracts that yielded significant differences (P < 0.05) are indicated by the different letters above each bar.

• 

  Fig. 4 Effect of peanut sprout extracts on leptin protein expression in adipose tissue of rats. Frozen adipose tissue was homogenized in lysis buffer. Protein concentration was determined by using a Bio-Rad method. Equal amounts of proteins (30 µg) were resolved by SDS-PAGE, transferred to the membranes and probed with leptin. Above photographs of chemiliuminiscent detection of the western blots, are shown and under the graph of quantitative analysis which were representatives of three independent experiments. Each bar represents the mean ± SE

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