Lab Anim Res.  2010 Jun;26(2):203-209.

The Effect of Quercetin on the BK(Ca) in Umbilical Cord Vein-derived Mesenchymal Stem Cells

Affiliations
  • 1Department of Physiology, College of Medicine, Seoul National University, Seoul, Korea.
  • 2Department of Physiology, College of Medicine, Chungbuk National University, Cheongju, Korea. yangmik@chungbuk.ac.kr

Abstract

Recently, we demonstrated that a large conductance Ca2+-activated K+ channel (BK(Ca)) in human umbilical cord vein-derived mesenchymal cells (hUC-MSCs). In this study, we studied effects of quercetin, the flavonoid used for the modulator of cell cycle and the treatment of anti-tumor, on BK(Ca) in hUC-MSCs using single channel and/or whole cell configuration. Single channel conductance was 277.8+/-36.2 pS in hUC-MSCs and the value is similar to that of typical BK(Ca) channel reported in other cells. The single channel current was activated by increasing of intracellular Ca2+. Quercetin (30~100 micrometer) increased BK(Ca) in whole cell patch configuration whereas EGCG was not affected on the channel. The activation effect of quercetin on BK(Ca) channel was also observed in inside-out configuration. The quercetin-induced BK(Ca) was increased a concentration-dependent manner with an IC50 value of 19.9 micrometer. Taken together, BK(Ca) in hUC-MSCs can be an important target for the action of quercetin and the channels are partly may contribute to maintaining homeostasis by regulation of K ion flux in hUC-MSCs.

Keyword

Human umbilical cord vein-derived mesenchymal cells; quercetin; BK(Ca)

MeSH Terms

Cell Cycle
Homeostasis
Humans
Inhibitory Concentration 50
Mesenchymal Stromal Cells
Quercetin
Umbilical Cord
Quercetin
Full Text Links
  • LAR
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error