J Korean Orthop Res Soc.  2006 Oct;9(2):188-194.

Difference in HLA-DR Expression of Human Umbilical Cord Blood Derived Mesenchymal Stem Cells after Tri-lineage Differentiation

• Baek GO
• Sung JH
• Moon JS
• Ha CW

Abstract

PURPOSE
The aim of this study was to examine the expression of HLA-DR surface antigen in undifferentiated human umbilical cord blood (hUCB) derived mesenchymal stem cells (MSCs) and after osteogenic, chondrogenic, and adipogenic differentiation.
MATERIALS AND METHODS
hUCB-derived MSCs were differentiated into osteogenic, chondrogenic, and adipogenic lineages. Differentiation was assessed by immunohistochemical staining and RT-PCR. The expression of HLA-DR was assessed with antihuman HLA-DR antibody in undifferentiated hUCB-derived MSCs and after tri-lineage differentiation.
RESULTS
HLA-DR expression was negative in undifferentiated hUCB-derived MSCs and after osteogenic and adipogenic differentiation. However, HLA-DR surface antigen was expressed after chondrogenic differentiation.
CONCLUSION
The immunologic properties of hUCB-derived MSCs differ from known reports on bone marrow derived MSCs from the results of this study. Careful immunological survey seems to be needed in case of considering the transplantation of hUCB-derived MSCs differentiated into chondrocytes or cartilaginous tissue.

Keyword

Human umbilical cord blood; Mesenchymal stem cells; Chondrogenic differentiation; Immunologic properties; HLA-DR

MeSH Terms

Antigens, Surface
Bone Marrow
Chondrocytes
Fetal Blood*
HLA-DR Antigens*
Humans*
Mesenchymal Stromal Cells*
Umbilical Cord*
Antigens, Surface
HLA-DR Antigens