Lab Anim Res.  2016 Jun;32(2):99-104. 10.5625/lar.2016.32.2.99.

Anti-Helicobacter pylori activity of crude N-acetylneuraminic acid isolated from glycomacropeptide of whey

Affiliations
  • 1Laboratory Animal Resource Center, Daegu Gyeongbuk Institute of Science & Technology (DGIST), Daegu, Korea.
  • 2Laboratory Animal Medicine and Brain Korea 21 PLUS Project, College of Veterinary Medicine, Chonnam National University, Gwangju, Korea. jonpark@jnu.ac.kr
  • 3Department of Biochemistry, College of Medicine, Konyang University, Daejeon, Korea.
  • 4Lifetree Biotechnology Institute, Lifetree Bitotech Co. Ltd., Gyeonggi, Korea.

Abstract

Helicobacter pylori colonizes the gastric mucosa of about half of the world's population, causing chronic gastritis and gastric cancer. An increasing emergence of antibiotic-resistant H. pylori arouses demand on alternative non-antibiotic-based therapies. In this study, we freshly prepared crude N-acetylneuraminic acid obtained from glycomacropeptide (G-NANA) of whey through a neuraminidase-mediated reaction and evaluated its antibacterial ability against H. pylori and H. felis. Overnight cultures of the H. pylori were diluted with fresh media and different concentrations (1-150 mg/mL) of crude G-NANA were added directly to the culture tube. Bacterial growth was evaluated by measuring the optical density of the culture medium and the number of viable bacteria was determined by a direct count of the colony forming units (CFU) on agar plates. For the in vivo study, mice were orally infected with 100 µL (5×108 cfu/mL) of H. felis four times at a day's interval, accompanied by a daily administration of crude G-NANA or vehicle. A day after the last infection, the mice were daily administered the crude G-NANA (0, 75, and 300 mg/mL) for 10 days and euthanized. Their stomachs were collected and bacterial colonization was determined by quantitative real-time PCR. Crude G-NANA inhibited H. pylori's growth and reduced the number of viable bacteria in a dose-dependent manner. Furthermore, crude G-NANA inhibited bacterial colonization in the mice. These results showed that crude G-NANA has antibacterial activity against Helicobacter and demonstrated its therapeutic potential for the prevention of chronic gastritis and gastric carcinogenesis induced by Helicobacter infection in humans.

Keyword

N-neuraminic acid; glycomacropeptide; Helicobacter; antibacterial activity

MeSH Terms

Agar
Animals
Bacteria
Carcinogenesis
Cats
Colon
Felis
Gastric Mucosa
Gastritis
Helicobacter
Helicobacter Infections
Helicobacter pylori
Humans
Mice
N-Acetylneuraminic Acid*
Real-Time Polymerase Chain Reaction
Stem Cells
Stomach
Stomach Neoplasms
Whey*
Agar
N-Acetylneuraminic Acid
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