Korean J Vet Res.  2014 Dec;54(4):225-231. 10.14405/kjvr.2014.54.4.225.

Localization of Klotho in cisplatin induced acute kidney failure

• Park SR
• Kim TW
• Kim YJ
• Kim HT
• Ryu SY
• Jung JY

• KMID: 2307887
• DOI: http://doi.org/10.14405/kjvr.2014.54.4.225

Abstract

Klotho deficiency is an early event in acute kidney injury (AKI) that exacerbates acute kidney damage. The present study explored the expression of Klotho and inflammation related factors in cisplatin-induced AKI. Rats (n = 18) were treated with cisplatin intraperitoneal injection (5 mg/kg) or left untreated as controls (n = 6), then sacrificed at 5 (n = 6) and 10 days (n = 6) treatment. Five days after cisplatin injection, the serum kidney enzymes and kidney cell apoptosis were significantly increased. Moreover, the expression of Klotho was decreased when compared to the control group, especially in the cortex and outer medulla regions. In contrast, inflammation related signals including nuclear factor kappa B, tumor necrosis factor-alpha, and tumor necrosis factor-like weak inducer of apoptosis were enhanced. However, 10 days after cisplatin injection, Klotho expression was enhanced upon both IHC and Western blot analysis, with slightly recovered renal function and decreased apoptosis. Furthermore, inflammation related signals expression was decreased relative to the 5 days group. Overall, this study confirmed the opposite expression patterns between Klotho and inflammation related signals and their localization in cisplatin-induced AKI kidney.

Keyword

acute kidney injury; inflammatory cytokine; Klotho; localization

MeSH Terms

Acute Kidney Injury*
Animals
Apoptosis
Blotting, Western
Cisplatin*
Inflammation
Injections, Intraperitoneal
Kidney
Necrosis
NF-kappa B
Rats
Tumor Necrosis Factor-alpha
Cisplatin
NF-kappa B
Tumor Necrosis Factor-alpha