Korean J Bone Metab.  2011 May;18(1):23-32.

Microarray Analysis of Gene Expression During Differentiation of Human Mesenchymal Stem Cells Treated with Vitamin E in vitro into Osteoblasts

• Ahn KH
• Jung HK
• Jung SE
• Yi KW
• Park HT
• Shin JH
• Kim YT
• Hur JY
• Kim SH
• Kim T

Abstract

OBJECTIVE
Supplementation with vitamin E is able to protect bone against free radical-induced elevation of bone-resorbing cytokines. We examined gene expression by microarray analysis during the differentiation of human mesenchymal stem cells treated with vitamin E into osteoblasts in vitro.
METHODS
Human bone marrow stem cells were cultured in osteogenic differentiation medium and vitamin E was added. A colorimetric immunoassay for the quantification of cell proliferation was used to measure osteoblast differentiation. Gene expression was analyzed using a microarray technique. We also used a real time reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS
It was found that vitamin E enhanced cell proliferation when compared to cells cultured in media without vitamin E. We focused on 68 genes which are related to osteogenesis and osteoclastogenesis. Alkaline phosphatase, transforming growth factor-beta 1, fibroblast growth factor receptor 1, matrix metalloproteinase 2, muscle segment homeobox 2, bone morphogenetic protein 1, biglycan, vascular endothelial growth factor B, dentin sialophosphoprotein, cartilage oligomeric matrix protein, runt-related transcription factor 2, fibroblast growth factor receptor 3, and SMAD2 were upregulated > 2-fold compared to the control. Conversely, osteopetrosis-associated transmembrane protein 1, microphthalmia-associated transcription factor, and epidermal growth factor receptor were downregulated > 2-fold compared to the control. Vitamin E produced a 1.5-fold increase in the expression of runt-related transcription factor 2 and transforming growth factor-beta 1 as determined by real time RT-PCR.
CONCLUSION
Vitamin E had a positive effect on the gene expressions regarding osteogenic differentiation of mesenchymal stem cells.

Keyword

Gene expression; Human bone marrow stem cells; Osteoblasts; Vitamin E

MeSH Terms

Alkaline Phosphatase
Biglycan
Bone Marrow
Bone Morphogenetic Protein 1
Cartilage
Cell Proliferation
Cytokines
Dentin
Durapatite
Extracellular Matrix Proteins
Gene Expression
Genes, Homeobox
Glycoproteins
Humans
Immunoassay
Matrix Metalloproteinase 2
Mesenchymal Stromal Cells
Microarray Analysis
Microphthalmia-Associated Transcription Factor
Muscles
Osteoblasts
Osteogenesis
Phosphoproteins
Receptor, Epidermal Growth Factor
Receptor, Fibroblast Growth Factor, Type 1
Receptor, Fibroblast Growth Factor, Type 3
Sialoglycoproteins
Stem Cells
Transcription Factors
Vascular Endothelial Growth Factor B
Vitamin E
Vitamins
Alkaline Phosphatase
Biglycan
Bone Morphogenetic Protein 1
Cytokines
Durapatite
Extracellular Matrix Proteins
Glycoproteins
Matrix Metalloproteinase 2
Microphthalmia-Associated Transcription Factor
Phosphoproteins
Receptor, Epidermal Growth Factor
Receptor, Fibroblast Growth Factor, Type 1
Receptor, Fibroblast Growth Factor, Type 3
Sialoglycoproteins
Transcription Factors
Vascular Endothelial Growth Factor B
Vitamin E
Vitamins