Korean J Androl.
1998 Dec;16(2):129-134.
Expression of Oscillin in Mouse Testis
- Affiliations
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- 1Department of Biology, College of Natural Sciences, Kyonggi University, Suwon, Korea.
- 2Biomed Institute, Suwon, Korea.
- 3Department of Biology, College of Natural Sciences, Hanyang University, Seoul, Korea.
Abstract
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PURPOSE: To verify the expression of the egg activator oscillin in mouse testis and adult organs.
MATERIALS AND METHODS
Genomic PCR using primers for oscillin was conducted to confirm that the PCR product was derived from cDNA. Total RNA isolated from developing, immature, and mature testis was subjected to RT-PCR and restriction analysis. In situ hybridization of adult testis was performed to localize the oscillin transcript using cRNA probe.
RESULTS
Genomic PCR using the primer for RT-PCR revealed no amplification product, suggesting that the oscillin gene consists of at least two exons. The RT-PCR product of oscillin mRAN was detected in testis beginning 2 weeks after birth. Oscillin mRAN was detected in both germ and somatic cells such as Sertoli and Leydig cells by in situ hybridization. The testis showed al high level of oscillin mRAN compared with other adult organs.
CONCLUSION
Oscillin is not a testis-specific transcript and therefore may have another function intracellularly as well ad serving as a trigger for egg activation.
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