J Vet Sci.  2007 Sep;8(3):209-212. 10.4142/jvs.2007.8.3.209.

The expression and cellular localization of phospholipase D isozymes in the developing mouse testis

• Kim S
• Kim H
• Lee Y
• Hyun JW
• Lee YH
• Shin MK
• Min DS
• Shin T

• KMID: 1090792
• DOI: http://doi.org/10.4142/jvs.2007.8.3.209

Abstract

To examine the involvement of phospholipase D (PLD)isozymes in postnatal testis development, the expression ofPLD1 and PLD2 was examined in the mouse testis atpostnatal weeks 1, 2, 4, and 8 using Western blot analysisand immunohistochemistry. The expression of both PLD1and PLD2 increased gradually with development frompostnatal week 1 to 8. Immunohistochemically, PLDimmunoreactivity was detected in some germ cells in thetestis and interstitial Leydig cells at postnatal week 1.PLD was mainly detected in the spermatocytes andresidual bodies of spermatids in the testis after 8 weeksafter birth. The intense immunostaining of PLD in Leydigcells remained unchanged by postnatal week 8. Thesefindings suggest that PLD isozymes are involved in thespermatogenesis of the mouse testis.

Keyword

cellular localization; mouse; phospholipase D; spermatogenesis; testis

MeSH Terms

Animals
Blotting, Western
Female
Immunohistochemistry
Isoenzymes
Male
Mice
Mice, Inbred BALB C
Phospholipase D/biosynthesis/*metabolism
Spermatogenesis/physiology
Testis/*enzymology/growth & development

Figure

• 

  Fig. 1 Increased expression of PLD1 and PLD2 protein in the mouse testis at 1, 2, 4, and 8 weeks after birth. Tissue homogenates were immunoprecipitated and immunoblotted with anti-PLD antibody. The data shown are representative of three independent experiments. The anti-PLD antibody specifically recognized PLD1 and PLD2. Molecular size markers are indicated on the left.

• 

  Fig. 2 Immunohistochemical localization of PLD in the testis at 1 (A), 2 (B), 4 (D), and 8 weeks (E) after birth. A and B: PLD immunostained undifferentiated cells in the seminiferous tubules (arrows). Some Leydig cells (asterisks) were immunopositive for PLD. C: Negative control. D: PLD immunostained some primary spermatocytes and intranuclear localization is evident (arrowheads). The asterisk indicates Leydig cells. E: PLD immunoreactivity was seen in some primary spermatocytes (arrowheads) and residual bodies of spermatids (arrows) as well as in Leydig cells (asterisk). Counterstain with hematoxylin. bar = 30 µm.

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