Korean J Urol.  2002 Dec;43(12):1086-1092.

The Change of Histologic Features and Eicosanoids Level in Rat Testicular Ischemia-Reperfusion Injury

• Lee SJ
• Lee GH
• Kim HJ
• Kim HG
• Kim EJ

Abstract

PURPOSE: It has been suggested that prostaglandins (PGs) play an important role in ischemia-reperfusion (IR) organ injury. However, the significance of PGs is not well documented for a testicular IR injury. The purpose of this study was to assess the potential role of PGs in rat testicular ischemia and IR injury
MATERIALS AND METHODS
Sprague-Dawley male rats were divided into 7 groups, each containing 7 rats. The right spermatic cord was clamped with vascular clamps for 30 minute (group B, C), 90 minute (D, E) and 240 minute (F, G). Groups B, D, and F were ischemia groups and the group C, E, and G were reperfusion groups. Reperfusion was carried out after ischemia lasting 30, 90 and 240 min. The levels of thromboxane B2 (TxB2), 6-keto-PGF1alpha and prostaglandins E2 (PGE2) were measured using High-Performance Liquid Chromatography (HPLC).
RESULTS
The levels of TxB2 were significantly elevated in groups D, E, F, and G than in the controls (p<0.05, each group). The TxB2 levels in the ischemia group F were significantly decreased compared to that of group D (p<0.05). The histological features shown in groups D, and F were typical ischemic changes, but atypical in groups B, and C. The most severe damage was noted in group G. These features were well matched with the changes of TxB2 in testicular ischemia and IR injury. The levels of 6-keto-PGF1alpha were significantly elevated in all the ischemia and IR groups compared to that of the controls (p<0.05).
CONCLUSIONS
These results suggested that TxB2 might have an active role in testicular IR injury, although PGE2 and 6-keto-PGF1alpha seemed not to have an active role.

Keyword

Testicular ischemia-reperfusion injury; Histologic features; Prostaglandins; HPLC

MeSH Terms

Animals
Chromatography, High Pressure Liquid
Chromatography, Liquid
Dinoprostone
Eicosanoids*
Humans
Ischemia
Male
Prostaglandins
Rats*
Rats, Sprague-Dawley
Reperfusion
Reperfusion Injury*
Spermatic Cord
Thromboxane B2
Dinoprostone
Eicosanoids
Prostaglandins
Thromboxane B2