Korean J Urol.  1993 Oct;34(5):770-777.

Effect of transforming growth factor ? on proliferation of prostatic cells

• Kim HJ
• Lee JD
• Ahn HJ

Abstract

Transforming-growth factor beta l, a prototypital autocrine growth factor, exhibits such a remarkable diversity of biological activity that may stimulate or inhibit cellular proliferation and stimulate extracellular matrix formation depending on the cell type. We evaluated the effect of TGF-beta1 on proliferation of the human prostatic epithelial cell and fibroblast. Epithelial cells were released from tissues by collagen digestion, and primary and subcultures cells were grown in PFMR-4A medium supplemented with epidermal growth factor, phosphoelhanolamine, cholera toxin, selenium, insulin, hydrocortisone, and bovine pituitary extract. Prostate-derived fibroblasts were cultured using RPMI 1640 medium with 10% fetal bovine serum. Verification of prostatic epithelial cells was accomplished by indirect immunofluorescence detection of prostate specific antigen, cytokeratin, and prostate-derived fibroblast was confirmed by immunofluorescence detection Of vimentin. Proliferation assay was performed using 3H-thymidine assay. Time and dose dependent inhibitory effects of TGF-beta1 on proliferation of prostatic epithelial cells were noted (86+/-6% at 0.1ng,ml and 21+/-3% at 10ng/ml after 96hr exposure). while TGF-beta1 at a high concentration stimulated the proliferation of prostate-derived tibroblasts (125+/-11% at lng/ml after 48hr exposure, 118+/-10% at 1ng/ ml arter 96hr exposure). However in the absence of fetal bovine serum, TGF-betal, at a physiologic range of 0.01 ng/ml showed inhibitory effect on proliferation of prostate-derived fibroblasts. These data show that TGF-beta1, a growth modulating cytokine, regulates growth of prostatic epithelial cells and prostate-derived fibroblast in the different way.

Keyword

TGF-beta1; Tissue culture; Prostate

MeSH Terms

Cell Proliferation
Cholera Toxin
Collagen
Digestion
Epidermal Growth Factor
Epithelial Cells
Extracellular Matrix
Fibroblasts
Fluorescent Antibody Technique
Fluorescent Antibody Technique, Indirect
Humans
Hydrocortisone
Insulin
Keratins
Prostate
Prostate-Specific Antigen
Selenium
Transforming Growth Factor beta1
Transforming Growth Factors*
Vimentin
Cholera Toxin
Collagen
Epidermal Growth Factor
Hydrocortisone
Insulin
Keratins
Prostate-Specific Antigen
Selenium
Transforming Growth Factor beta1
Transforming Growth Factors
Vimentin