J Korean Ophthalmol Soc.  1998 Jun;39(6):1192-1203.

The Effects of TGF-beta2 and bFGF on the Proliferation of Retinal Pigment Epithelial Cells

• Yoon HS
• Roh SH
• Lee SC
• Jeong JH
• Yoo YH

Abstract

This study was undertaken to document the effect of transforming growth factor-beta2 (TGF-beta2(TGF-beta2) and basic fibroblast growth factor (bFGF) on the proliferation of pig retinal pigment epithelial cells (RPE). Whereas bFGF increased the proliferation, TGF-beta2 showed the inhibitory effect on the proliferation The inhibitory effect of TGF-beta2 disappeared in RPE subcultured with 10ng/ml of bFGF. Both TGF-beta2- and bFGF-specific antisense oligonucleotides blocked the autocrine effect of the growth factors. PLC-71 -specific antisense oligonucleotide inhibited the effect of TGF-beta2 and bFGF. Genistein inhibited the effect of TGF-beta2 and bFGF in dose-dependent man, ner. The data suggest the involvement. of in PLC-/1 and tyrosine kinase in signalling.

Keyword

bFGF; PLC-yl; retinal pigment epithelial cell; TGF-beta2; tyro sine kinase

MeSH Terms

Epithelial Cells*
Fibroblast Growth Factor 2
Genistein
Intercellular Signaling Peptides and Proteins
Oligonucleotides, Antisense
Protein-Tyrosine Kinases
Retinaldehyde*
Transforming Growth Factor beta2*
Fibroblast Growth Factor 2
Genistein
Intercellular Signaling Peptides and Proteins
Oligonucleotides, Antisense
Protein-Tyrosine Kinases
Retinaldehyde
Transforming Growth Factor beta2