J Bone Metab.  2013 Nov;20(2):61-65. 10.11005/jbm.2013.20.2.61.

Association of Bone Marrow Sphingosine 1-phosphate Levels with Osteoporotic Hip Fractures

• Ahn SH
• Koh JM
• Gong EJ
• Byun S
• Lee SY
• Kim BJ
• Lee SH
• Chang JS
• Kim GS

• KMID: 2286295
• DOI: http://doi.org/10.11005/jbm.2013.20.2.61

Abstract

BACKGROUND
Sphingosine 1-phosphate (S1P) has been discovered to be a critical regulator of bone metabolism. Very recently, we found that higher circulating S1P levels were associated with higher rate of prevalent osteoporotic fracture in human.
METHODS
This was a cross-sectional study of 16 patients who underwent hip replacement surgeries. Bone marrow fluids were obtained during hip surgeries, and the S1P levels were measured using a competitive enzyme-linked immunosorbent assay (ELISA) assay. Bone mineral densities (BMDs) at various skeletal sites were obtained using dual energy X-ray absorptiometry.
RESULTS
Among 16 patients, 4 patients were undergone operations due to hip fractures, and the others were done by any other causes. Bone marrow S1P levels were significantly lower in patients with hip fractures than in those without, before and after adjusting for confounding factors (P=0.047 and 0.025, respectively). We failed to demonstrate significant associations between bone marrow S1P levels and any BMD values (gamma=0.026-0.482, P=0.171-0.944).
CONCLUSIONS
In conjunction with our previous findings, these suggest that the effects of gradient between peripheral blood and bone marrow, but not S1P itself, may be the most critical on bone metabolism.

Keyword

Bone density; Osteoporotic fracture; Sphingosine 1-phosphate

MeSH Terms

Blood
Bone Density
Bone Marrow*
Cross-Sectional Studies
Enzyme-Linked Immunosorbent Assay
Hip Fractures*
Hip*
Humans
Lysophospholipids
Metabolism
Osteoporotic Fractures
Sphingosine*
Lysophospholipids
Sphingosine

Figure

• 

  Fig. 1 Bone marrow sphingosine 1-phosphate (S1P) levels according to the osteoporotic hip fracture status were investigated (A) before and (B) after adjusting for confounding factors such as age and sex.

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