Korean J Physiol Pharmacol.  2010 Aug;14(4):199-204. 10.4196/kjpp.2010.14.4.199.

The Efficacy of Shikonin on Cartilage Protection in a Mouse Model of Rheumatoid Arthritis

Affiliations
  • 1Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration, Eumseong 369-873, Korea.
  • 2Department of Rheumatology, School of Medicine, Kyung Hee University, Seoul 130-701, Korea.
  • 3Department of Clinical Pharmacology, School of Medicine, Kyung Hee University, Seoul 130-701, Korea. ysvin@khu.ac.kr

Abstract

The potential therapeutic action of shikonin in an experimental model of rheumatoid arthritis (RA) was investigated. As a RA animal model, DBA/1J mice were immunized two times with type II collagen. After the second collagen immunization, mice were orally administered shikonin (2 mg/kg) once a day for 35 days, and the incidence, clinical score, bone mineral density (BMD), bone mineral content (BMC) and joint histopathology were evaluated. BMD in the proximal regions of the tibia largely increased in the shikonin treatment group compared with the control group. We also examined the effect of shikonin on inflammatory cytokines and cartilage protection. Shikonin treatment significantly reduced the incidence and severity of collagen-induced arthritis (CIA), markedly abrogating joint swelling and cartilage destruction. Shikonin also significantly inhibited the production of matrix metalloproteinase (MMP)-1 and up-regulated tissue inhibitors of metalloproteinase (TIMP)-1 in mice with CIA. In conclusion, shikonin exerted therapeutic effects through regulation of MMP/TIMP; these results suggest that shikonin is an outstanding candidate as a cartilage protective medicine for RA.

Keyword

Shikonin; Bone mineral density; Bone mineral contents; MMP-1; TIMP-1

MeSH Terms

Animals
Arthritis, Experimental
Arthritis, Rheumatoid
Bone Density
Cartilage
Collagen
Collagen Type II
Cytokines
Immunization
Incidence
Joints
Mice
Models, Animal
Models, Theoretical
Naphthoquinones
Tibia
Tissue Inhibitor of Metalloproteinase-1
Collagen
Collagen Type II
Cytokines
Naphthoquinones
Tissue Inhibitor of Metalloproteinase-1

Figure

  • Fig. 1. Structure of shikonin.

  • Fig. 2. Experimental schedule.

  • Fig. 3. Histopathologies of hind paw sections from mice treated with shikonin. (A) Hematoxylin eosin staining of 2 mg/kg shikonintreated mice, (B) vehicle-treated mice, (C) 50 mg/kg Meloxicamtreated mice, (D) Normal group (non RA). ∗Pannus formation over articular cartilage.

  • Fig. 4. Induction of paw edema and effect of shikonin treatment (2 mg/kg) after 2nd immunization. Paw edema measured in type II collagen-induced arthritic mice (n=10). Treated with vehicle (♦), Meloxicam (50 mg/kg ×), and shikonin (2 mg/kg ▪) after 2nd immunization. ∗∗p< 0.005 and ∗∗∗p< 0.001 compared with vehicle-treated mice with CIA.

  • Fig. 5. Inhibitory effect of shikonin on disease progression in mice with collagen-induced arthritis (CIA). CIA was induced by primary (day 0) and secondary (day 20) immunizations with bovine type II collagen in Freund's complete adjuvant. (A) Arthritis score (clinical severity of arthritis). The maximum possible score is 16, as described in materials and methods. (B) Incidence of arthritis. Values are reported as the mean ± S.E.M. ∗p<0.05, ∗∗p< 0.005 and ∗∗∗p< 0.001 compared with vehicle-treated mice with CIA. Treated with vehicle (▪), Meloxicam (50 mg/kg ), and shikonin (2 mg/kg ▴).

  • Fig. 6. Effect of shikonin on IL-1β and TNF-α levels (pg/mg protein, mean±S.E.M) Serum was obtained from four groups of mice: sham group (no arthritis + vehicle treatment, n=10), control group (arthritis + vehicle, n=10), meloxicam group (arthritis + meloxicam treatment at 50 mg/kg/day n=10), and shikonin group (arthritis + shikonin treatment at 2 mg/kg/day n=10). After shikonin treatment, serum IL-1β and TNF-α decreased significantly, ∗∗∗p< 0.001, compared to the vehicle control group.

  • Fig. 7. The bone mineral density and bone mineral contents of tibia in vehicle, shikonin, and Meloxicam-treated arthritic mice. Values are reported as the mean±S.E.M. ∗p<0.05, ∗∗p<0.005 and ∗∗∗p<0.001, compared with vehicle-treated mice with CIA.


Cited by  1 articles

Dynamic Frequency of Blood CD4+CD25+ Regulatory T Cells in Rats with Collagen-induced Arthritis
Tingyu Wang, Jun Li, Zhigui Jin, Feihua Wu, Yunwu Li, Xiaohua Wang, Hong Zhou, Qian Zhou
Korean J Physiol Pharmacol. 2015;19(2):83-88.    doi: 10.4196/kjpp.2015.19.2.83.


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