Korean J Pediatr.  2009 May;52(5):567-575. 10.3345/kjp.2009.52.5.567.

Proteomic analysis of human serum from patients with temporal lobe epilepsy

• Lee CW
• Yu ST
• Choi HY
• Koh BJ
• Kwak YG

• KMID: 2281460
• DOI: http://doi.org/10.3345/kjp.2009.52.5.567

Abstract

PURPOSE
Epilepsy affects more than 0.5% of the world's population. It has a large genetic component and is caused by electrical hyperexcitability in the central nervous system. Despite its prevalence, the disease lacks definitive diagnostic serological biomarkers. To identify potential biomarkers for epilepsy by a convenient method, we analyzed the expression of serum proteins, reflecting alterations in the patient's proteomes. METHODS: We compared two-dimensional electrophoretic band patterns of human sera from eight patients with temporal lobe epilepsy (TLE) with those of eight control subjects. The differentially expressed bands were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and electrospray ionization quadrupole time-of-flight mass spectrometry. RESULTS: Twelve proteins were differentially expressed in the TLE group, of which 6 were identified. Expression of haptoglobin Hp2, PRO2675, immunoglobulin heavy chain constant region gamma 2, an unnamed protein, and three unidentified proteins were upregulated in serum from the patients with TLE, whereas those of major histocompatibility complex (MHC) class I antigen, plasma retinol-binding protein precursor, and three unidentified proteins were downregulated in these patients. After resection of the epileptogenic zone, the expressions of MHC class I antigen, immunoglobulin heavy chain constant region gamma 2, two of the downregulated unidentified proteins, and one of the upregulated unidentified proteins returned to the normal range. CONCLUSIONS: The 12 serum proteins in this study are potentially useful biomarkers for the diagnosis and monitoring of TLE.

Keyword

Proteomics; Proteome; Epilepsy; Temporal Lobe

MeSH Terms

Biomarkers
Blood Proteins
Central Nervous System
Epilepsy
Epilepsy, Temporal Lobe
Haptoglobins
Humans
Immunoglobulin Heavy Chains
Major Histocompatibility Complex
Mass Spectrometry
Plasma
Prevalence
Proteins
Proteome
Proteomics
Reference Values
Temporal Lobe
Blood Proteins
Haptoglobins
Immunoglobulin Heavy Chains
Proteins
Proteome