Korean J Pediatr.
2009 Sep;52(9):1015-1020. 10.3345/kjp.2009.52.9.1015.
Toll-like receptor 9 expression and interferon-alpha secretion upon CpG-ODN stimulation in allergic subjects
- Affiliations
-
- 1Department of Pediatrics, CHA University School of Medicine, Seongnam, Korea.
- 2Department of Internal Medicine, CHA University School of Medicine, Seongnam, Korea.
- 3Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea. kekim@yuhs.ac
- KMID: 2281257
- DOI: http://doi.org/10.3345/kjp.2009.52.9.1015
Abstract
- PURPOSE
The aim of this study is to explore the effect of the Toll-like receptor 9 (TLR9) expressed in plasmacytoid dendritic cells (pDCs) that respond to antigen to Th2 immune deviation in allergic patients.
METHODS
Subjects consisted of 19 allergic patients and 17 healthy volunteers. Skin prick tests and nasal provocation tests were performed for the two groups. Peripheral blood mononuclear cells (PBMCs) were collected from subjects and analyzed for the Lineage Cocktail (CD3, CD14, CD16, CD19, CD20, CD56) (-), HLA-DR (+), and CD123 (+) using flow cytometry. In addition, we analyzed TLR9 mRNA by reverse transcriptase-polymerase chain reaction. The level of interferon-alpha (IFN-alpha) of the PBMCs following stimulation with the TLR9 ligand CpG-ODN 2216 was also evaluated.
RESULTS
Analyses of CD123 (+) revealed a nearly similar distribution for the classical pDC markers in the allergic group (0.1%+/-0.04%) and in the controls (0.25%+/-0.23%). The mRNA levels of TLR9 on PBMCs were not different between the allergic group and the controls (1.29+/-0.41 vs. 1.25+/-0.23, respectively). Additionally, the level of IFN-alpha in PBMCs exposed to stimuli of the TLR9 ligand CpG-ODN 2216 was not significantly different between the two groups (911+/-829 vs. 1,095+/-888 pg/mL, respectively).
CONCLUSIONS
We found no evidence that TLR9-dependent immune responses in human pDCs are associated with allergic status.
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