Korean J Pediatr.  2004 Dec;47(12):1325-1333.

The Effects of Intravenous Immunoglobulin(IVIG) and Methylprednisolone on the mRNAs Expressions of VEGF, VCAM-1 and IL-1beta of Human Umbilical Vein Endothelial Cells(HUVEC) Stimulated by IL-1beta

• Kim SY
• Lim SJ
• Han JW
• Lee KY
• Lee JS

Abstract

PURPOSE
Kawasaki disease(KD) manifests a systemic vasculitis of unknown etiology in young children. Vascular endothelial growth factor(VEGF), vascular cell adhesion molecule-1(VCAM-1) and interleukin-1 beta(IL-1beta) may play important roles in the pathogenesis of KD. Intravenous immunoglobulin(IVIG) and methylprednisolone(MP) are therapeutically effective for KD, however, the precise mechanisms of the two drugs are still unknown. We investigated the therapeutic efficacy of IVIG and/or MP for KD in vitro.
METHODS
Human umbilical vein endothelial cells(HUVEC) obtained from umbilical cords of healthy newborns were cultured. After HUVEC were treated with IL-1beta, the effect of IVIG and/or MP on the in vitro activation of HUVEC were assessed by cell proliferation and reverse transcription-polymerase chain reaction-detected expression of mRNA coding for VEGF, VCAM-1, and IL-1beta.
RESULTS
IVIG and MP down-regulated the expression of VEGF mRNA induced by IL-1beta(P<0.05, respectively) significantly. The combination of both showed a synergistic effect on the expressions with a dose dependent manner of MP compared to the IVIG or MP alone respectively(P<0.05). IVIG and MP down-regulated the expression of VCAM-1 mRNA induced by IL-1beta(P<0.05, respectively). The combination of both showed a synergistic effect on the expressions with a dose dependent manner of MP(P<0.05). IVIG and MP down-regulated the expression of IL-1beta mRNA induced by IL-1beta(P<0.001, P<0.05, respectively). The combination of both showed a synergistic effect on the expressions with a dose dependent manner of MP(P<0.001).
CONCLUSION
These results suggested that IVIG and MP are therapeutically effective for KD in vitro as well as in vivo.

Keyword

Kawasaki disease; Human umbilical vein endothelial cells; Intravenous immunoglobulin; methylprednisolone; Vascular endothelial growth factor; Vascular cell adhesion molecule-1; Interleukin-1beta

MeSH Terms

Cell Adhesion
Cell Proliferation
Child
Clinical Coding
Human Umbilical Vein Endothelial Cells
Humans*
Immunoglobulins, Intravenous
Infant, Newborn
Interleukin-1
Interleukin-1beta
Methylprednisolone*
Mucocutaneous Lymph Node Syndrome
RNA, Messenger*
Systemic Vasculitis
Umbilical Cord
Umbilical Veins*
Vascular Cell Adhesion Molecule-1*
Vascular Endothelial Growth Factor A*
Immunoglobulins, Intravenous
Interleukin-1
Interleukin-1beta
Methylprednisolone
RNA, Messenger
Vascular Cell Adhesion Molecule-1
Vascular Endothelial Growth Factor A