Tuberc Respir Dis.  1999 Mar;46(3):338-349.

The expression of IL-8 and GROalpha/MGSA in HUVEC stimulated by the TNF-alpha and IL-1

• Song JS
• Shin MS
• Ahn JH
• Moon HS
• Park SH

Abstract

Polymorphonuclear leukocytes(PMN) are the predominant inflammatory cells recruited in acute lung injury such as adult respiratory distress syndrome, pneumonia and also chronic lung disease such as idiopathic pulmonary fibrosis and pulmonary emphysema. Interleukin -8(IL-8) is an 8,000 D protein produced by many cells and has potent neutrophil chemoattractant and activating properties. The GRO, also called melanoma growth-stimulatory activity(MGSA), referring to a peptide of 73 amino acids, was reported to be mitogenic for cultured human melanoma cells. Mature GRO/MGSA has marked sequence similarity to IL-8. In view of the structural similarities to IL-8, it was of particular interest to test GRO for neutrophil activating and chemotactic properties. We found a significant release of IL-8 and GRO/MGSA from the cultured human umbilical vein endothelial cell(HUVEC) which was stimulated either with TNFalpha or IL-1beta and also found the expression of IL-8 and GRO/MGSA mRNA. Neutrophil chemotactic activity was enhanced in accordance with the increased IL-8 and GRO/MGSA. Our study also suggest that the IL-8 is more important in the increased neutrophil chemotactic activity than GRO/MGSA when endothelial cell is stimulated with TNFalpha or IL-1beta in vitro.

Keyword

IL-8; GRO/MGSA; umbilical vein endothelial cell(HUVEC)

MeSH Terms

Acute Lung Injury
Amino Acids
Endothelial Cells
Humans
Idiopathic Pulmonary Fibrosis
Interleukin-1*
Interleukin-8*
Interleukins
Lung Diseases
Melanoma
Neutrophils
Pneumonia
Pulmonary Emphysema
Respiratory Distress Syndrome, Adult
RNA, Messenger
Tumor Necrosis Factor-alpha*
Umbilical Veins
Amino Acids
Interleukin-1
Interleukin-8
Interleukins
RNA, Messenger
Tumor Necrosis Factor-alpha