Korean J Pediatr.
2007 Feb;50(2):151-156. 10.3345/kjp.2007.50.2.151.
A study of serotyping of Streptococcus pneumoniae by multibead assay
- Affiliations
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- 1Department of Pediatrics, College of Medicine Ewha Woman's University, Korea. kaykim@ewha.ac.kr
- 2Department of Laboratory Medicine, College of Medicine Ewha Woman's University, Korea.
- 3Department of Pediatrics, College of Medicine, Seoul National University, Korea.
- KMID: 2278718
- DOI: http://doi.org/10.3345/kjp.2007.50.2.151
Abstract
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PURPOSE: Streptococcus pneumoniae is a major etiologic agent for pneumonia, meningitis, otitis media, and sepsis among young children. Multi-drug resistant strains have raised great concern worldwide, thus the importance of prevention with vaccines has been emphasized. However, vaccines may force the appearance of pneumococcal infections by nonvaccine serotypes. Thus, distribution of pneumococcal serotypes should be monitored to estimate vaccine efficacy. We used a new and efficient multibead assay in determining pnemococcal serotypes.
METHODS
From January to February 2005, 643 children were recruited from ten day care centers to isolate pneumococci from their oropharynx. Pneumococcal serotyping was performed on 62 pneumococcal isolates from 60 children by multibead assay. This immunoassay required two sets of latex particles coated with pneumococcal polysaccharides and serotype-specific antibodies. Twenty four newly developed monoclonal antibodies specific for common serotypes and a pool of polyclonal rabbit sera for some of the less common serotypes were used.
RESULTS
The most prevalent pneumococcal serotypes were serotype 6A, 19A, 19F, 23F, and 11A/ D/F which accounted more than 50 precent of all the 62 pneumococcal isolates. We found that multibead assay can be performed very rapidly and objectively.
CONCLUSION
This multibead immunoassay was very useful in serotyping clinical isolates of S. pneumoniae because it was simple, reliable and fast.
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