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Concordance of Three Automated Procalcitonin Immunoassays at Medical Decision Points

Cho HW, Kim SH, Cho Y, Jeong SH, Lee SG

Procalcitonin (PCT) is a useful bacterial infection biomarker with the potential for guiding antibiotic therapy. We evaluated the concordance of three automated PCT immunoassays: Kryptor (BRAHMS GmbH, Hennigsdorf, Germany), Atellica...
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Diagnostic Accuracy of the Quidel Sofia Rapid Influenza Fluorescent Immunoassay in Patients with Influenza-like Illness: A Systematic Review and Meta-analysis

Lee J, Song JU, Kim YH

Background: Although the Quidel Sofia rapid influenza fluorescent immunoassay (FIA) is widely used to identify influenza A and B, the diagnostic accuracy of this test remains unclear. Thus, the objective...
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Evaluation of Two Commercial Kits for Rapid Detection and Typing of Carbapenemase in Carbapenem-Resistant Enterobacterales

Lee S, Hur KH, Chung Y, Sung H, Kim MN

Background: Rapid detection of carbapenemase-producing Enterobacterales (CPE) is desirable to guide antimicrobial therapy and infection control. The NG-Test Carba5 (Carba5;NG Biotech, France) rapid multiplex lateral flow immunoassay and BD MAX...
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Spurious Thyroid Function Test Results due to Biotin Interference: a Report of Three Cases and a Literature Review

Park JY, Choi W, Yoon JH, Kang HC

Biotin (vitamin B7) is a water-soluble vitamin used as a co-enzyme for carboxylases essential for human metabolism. The high affinity to streptavidin makes biotin an important substance in immunoassays. Excessive...
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Positivity of SARS-CoV-2 Antibodies among Korean Healthy Healthcare Workers 1 and 2 Weeks after Second Dose of Pfizer-BioNTech Vaccination

Kim N, Minn D, Park S, Roh EY, Yoon JH, Park H, Shin S

The antibody titer of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was observed in 289 healthy healthcare workers who had completed the second dose of the Pfizer-BioNTech coronavirus disease...
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Comparative Evaluation of the STANDARD F Influenza A/B FIA Test with the Sofia Influenza A+B FIA and SD BIOLINE Influenza Ag A/B/A(H1N1) tests for Influenza A Virus Detection

Yoo S, Kim SH, Jeon GW, Park YH, Song SA, Shin JH

Background: This study aimed to evaluate the diagnostic performance of the STANDARD F Influenza A/B FIA test (SD Biosensor Inc., Korea) for the rapid detection of influenza A virus in...
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Comparison of Serologic Response of Hospitalized COVID-19 Patients Using 8 Immunoassays

Lee SM, Kim IS, Lim S, Lee SJ, Kim WJ, Shin KH, Moon SY, Chang CL

Background: In Korea, there were issues regarding the use of immunoassays for anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies to detect infection. So, we compared antibody results of eight...
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A Sensitive and Specific Liquid ChromatographyTandem Mass Spectrometry Assay for Simultaneous Quantification of Salivary Melatonin and Cortisol: Development and Comparison With Immunoassays

Shin S, Oh H, Park HR, Joo EY, Lee SY

Melatonin and cortisol are clinically important for diagnosing sleep and mood disorders. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/ MS) assay for simultaneous measurement of salivary melatonin and...
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Diagnostic Algorithm for the Rapid and CostEffective Detection of Clostridioides difficile Infection: Comparison between C. DIFF QUIK CHEK COMPLETE and VIDAS GDH & Toxin Assay

Yoo SJ, Whang DH

Background: We evaluated the analytical performance and cost-effectiveness of lateral flow immunoassays (LFIAs) in detecting Clostridioides difficile glutamate dehydrogenase (GDH) antigen and toxin A/B, followed by a rapid nucleic...
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Performance Evaluation of a Point of Care SelexOnTM B-Type Natriuretic Peptide Immunoassay

Jekarl DW, Lee S, Choi H, Cho SW, Park Hi

Background: This study was conducted to evaluate the analytical performance of the SelexOnTM B-type natriuretic peptide (BNP) assay (Osang Healthcare Inc., Korea), a new rapid lateral flow immunoassay for point...
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Cortisol Measurements in Cushing's Syndrome: Immunoassay or Mass Spectrometry?

Casals G, Hanzu FA

Determination of cortisol levels in the urine (24 hours urine free cortisol), saliva (late-night), or serum (total cortisol after dexamethasone suppression) is recommended to screen for Cushing's syndrome (CS). This...
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Validation of a Multiplex Assay for Measuring Specific IgG4

Kim SR, Park KH, Lee JE, Kim BJ, Lim KJ, Park JW

Purpose: Specific IgG4 (sIgG4) increases with allergen specific immunotherapy and may reflect a state of immune tolerance in food allergy. While ImmunoCAP® has been widely used to measure sIgG4 to...
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Report of the Korean Association of External Quality Assessment Service on Hepatitis Serology (2018–2019)

Cho EJ, Choi AR, Ryu JH, Yun SJ, Oh EJ

As a diagnostic immunology program of the Korean Association of External Quality Assessment Service, two trials were organized to assess the external quality of hepatitis serology in 2018 and 2019....
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Evaluation of a Newly Developed Rapid Automated Fluorescent Lateral Flow Immunoassay to Detect IgG and IgM Antibodies to Chikungunya Virus

Lee H, Ryu JH, Yun S, Jang JH, Choi AR, Cho SY, Park C, Lee DG, Oh EJ

The ichroma Chikungunya virus (CHIKV) IgG/IgM (Boditech Med Inc., Chuncheon, Korea) is a newly developed rapid lateral flow immunoassay for detection of anti- CHIKV-IgG/ IgM. This study conducted with thirty-six...
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Comparison of Clinical Utility between Phadiatop Assay and AdvanSureTM AlloScreen

Kim MK, Kwon MJ, Park H, Woo HY

Background: Multiple Allergo-Sorbent Test (MAST) allows simultaneous detection of specific IgE antibodies using multiple allergens, and it is commonly used for allergy screening. Phadiatop assay (Phadia AB, Sweden), including Phadiatop...
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Distinct Inflammation Biomarkers in Healthy Individuals and Patients with Schizophrenia: A Reliability Testing of Multiplex Cytokine Immunoassay by Bland-Altman Analysis

Yeh TC, Chu HT, Tsai CK, Chang HA, Yang FC, Huang SY, Liang CS

OBJECTIVE: Since the inflammatory process has been implicated in the pathophysiology of psychiatric disorder, an important issue emerging is to assess the test-retest reliability of cytokine measurement in healthy individuals...
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Comparison of Six Automated Immunoassays With Isotope-Diluted Liquid Chromatography-Tandem Mass Spectrometry for Total Thyroxine Measurement

Yu S, Zhou W, Cheng X, Meng Q, Li H, Hou L, Lu J, Xie S, Cheng Q, Zhang C, Qiu L

BACKGROUND: Accurate serum total thyroxine (TT4) measurement is important for thyroid disorder diagnosis and management. We compared the performance of six automated immunoassays with that of isotope-diluted liquid chromatography-tandem mass...
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Validation of PROTIA™ Allergy-Q 64 Atopy® as a Specific IgE Measurement Assay for 10 Major Allergen Components

Kim SR, Park KH, Lee JH, Kim BJ, Hwang JH, Lim KJ, Park JW

PURPOSE: Component-resolved diagnostics (CRD) is expected to provide additional diagnostic information in allergic patients. PROTIA™ Allergy-Q 64 Atopy®, a recently developed CRD-based multiplex specific immunoglobulin E (sIgE) assay, can quantitatively...
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Laboratory Diagnosis of Clostridium difficile Infection in Korea: The First National Survey

Chung HS, Park JS, Shin BM

In May 2015, we conducted a voluntary online survey on laboratory diagnostic assays for Clostridium difficile infection (CDI) across clinical microbiology laboratories in Korea. Responses were obtained from 66 laboratories,...
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The novel latex agglutination turbidimetric immunoassay system for simultaneous measurements of calprotectin and hemoglobin in feces

Hiraoka S, Takashima , Inokuchi T, Nakarai A, Takahara M, Harada K, Seki Y, Watanabe K, Kato J, Okada H

BACKGROUND/AIMS: Fecal calprotectin (Fcal) as well as the fecal immunochemical test (FIT) are useful biomarkers for detecting activity and mucosal healing in inflammatory bowel diseases. Here, we report the performance...
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