Korean J Otorhinolaryngol-Head Neck Surg.  2008 Jan;51(1):51-57.

The Differential Expression of Upper and Lower Airway after Lipopolysaccharide Administration in a Murine Allergy Model

• Park SK
• Rhee CS
• Oh SH
• Kim YK
• Cho SH
• Min KU
• Kim YY

Abstract

BACKGROUND AND OBJECTIVES: It is well recognized that respiratory infection modulates allergic airway inflammation. However, there is apparent controversy about the role for exposure site of bacterial components such as lipopolysaccharide (LPS) in allergic inflammation. This study investigated allergic airway inflammation after the administration of the same amount of LPS in the ovalbumin (OVA) induced murine allergy model. MATERIALS AND METHOD: Six-week-old BALB/c mice were sensitized with OVA at day 0 and 7 of the start of experiment via intraperitoneal route and challenged with OVA or OVA and LPS at day 14, 15, 16, 21, 22 and 23 with or without anesthesia via intranasal route. After the last challenge, parameters for airway hyperresponsiveness were measured, followed by evaluation of airway histology, serum OVA specific antibodies, inflammatory cells and cytokines of bronchoalveolar lavage fluid (BALF) and nasal cavity lavage fluid (NALF).
RESULTS
Airway hyperresponsiveness significantly increased not only in the groups of asthma (ASM) and asthma with LPS (ASM+LPS) but also in the allergic rhinitis with LPS (AR+LPS) group. In the lung, infiltration of inflammatory cells including eosinophils and lymphocytes were prominent only in the groups of ASM and ASM+LPS. When compared with the control, in the nasal cavity, the infiltration of inflammatory cells was prominent in all of the groups, whereas in BALF, IL-5 and IL-13 were significantly increased in the groups of ASM+LPS and ASM, and OVA specific IgE and IgG2a were significantly increased in all of the groups.
CONCLUSION
These results support the concept that the systemic mechanism linking allergic rhinitis to asthma depends on the location and extent of airway allergen exposure.

Keyword

Allergy; Lipopolysaccharide; Airway; Inflammation

MeSH Terms

Anesthesia
Animals
Antibodies
Asthma
Bronchoalveolar Lavage Fluid
Cytokines
Eosinophils
Hypersensitivity
Immunoglobulin E
Immunoglobulin G
Inflammation
Interleukin-13
Interleukin-5
Lung
Lymphocytes
Mice
Nasal Cavity
Ovalbumin
Ovum
Rhinitis
Rhinitis, Allergic, Perennial
Therapeutic Irrigation
Antibodies
Cytokines
Immunoglobulin E
Immunoglobulin G
Interleukin-13
Interleukin-5
Ovalbumin