Korean J Pathol.  2005 Feb;39(1):9-14.

Microsatellite Instability and Mismatch Repair Protein (hMLH1, hMSH2) Expression in Intrahepatic Cholangiocarcinoma

Affiliations
  • 1Department of Pathology, Inje University Seoul Paik Hospital, Seoul, Korea. jadepaka@hanmail.net
  • 2Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.

Abstract

BACKGROUND: To clarify the role of the mismatch repair (MMR) system in the carcinogenesis of intrahepatic cholangiocarcinoma (ICC), we investigated the microsatellite instability (MSI) status and MMR protein expression in ICC.
METHODS
Thirty-six cases of ICCs were examined by microsatellite analysis for 55 markers that encompassed all of the chromosomal arms and BAT26. An immunohistochemical study for hMLH1 and hMSH2 was also performed.
RESULTS
Widespread MSI (MSI-H) accompanied with a loss of hMLH1 expression was found in one case (2.8%). This MSI-H case was an adenosquamous carcinoma showing intraductal tubulopapillary adenocarcinoma and invasive adenosquamous carcinoma component. Loss of hMLH1 was noted in both components while the frequency and shifted band patterns of MSI were not identical between the components. Another 10 ICCs (27.8%) revealed low level MSI with preserved MMR gene expression.
CONCLUSIONS
Our data suggested that a genetic defect in the MMR system and MSI is not a major pathway in the carcinogenesis of ICC.

Keyword

Cholangiocarcinoma; Microsatellite repeats; hMLH1 protein; hMSH2 protein; Immunohistochemistry

MeSH Terms

Adenocarcinoma
Arm
Carcinogenesis
Carcinoma, Adenosquamous
Cholangiocarcinoma*
DNA Mismatch Repair*
Gene Expression
Immunohistochemistry
Microsatellite Instability*
Microsatellite Repeats*
Full Text Links
  • KJP
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error