Korean J Pathol.  2007 Dec;41(6):380-386.

Analysis of Microsatellite Instability in Ovarian Epithelial Cancer

• Sol MY
• Choi KU
• Kim JY
• Kang HJ
• Shin DH
• Kim ID
• Choi HS
• Seo SJ

Abstract

BACKGROUND: The aim of this study was to clarify the incidence and role of microsatellite instability (MSI) in sporadic ovarian epithelial cancers (OEC). We investigated the MSI status and mismatch repair (MMR) protein expression in OEC.
METHODS
MSI was examined by fluorescence- based polymerase chain reaction using five NCI panel markers (BAT25, BAT26, D2S123, D5S346 and D17S250) in 46 cases of OEC. Immunohistochemistry (IHC) for hMLH1 and hMSH2 was performed.
RESULTS
Seven cases (15.2%) exhibited high-frequency MSI (MSIH), one exhibited low-frequency MSI (MSI-L), and the remaining 38 demonstrated microsatellite stability (MSS). MSI-H in OEC was not associated with histologic grade, FIGO stage, tumor size, mitoses or histologic type. Loss of expression of either hMLH1 or hMSH2 was observed in 4 of the 7 (59.3%) MSI-H cases, whereas 4 of the 39 (10.3%) MSI-L or MSS tumors revealed loss of expression of MMR proteins. The sensitivity and specificity of immunohistochemistry for hMLH1 and hMSH2 were 57.1% and 89.7%.
CONCLUSIONS
Our data suggest that a genetic defect in the MMR system might play a role in the carcinogenesis of a minor subset of sporadic OEC however, immunohistochemical testing for hMLH1 and hMSH2 cannot accurately determine microsatellite instability status in OEC.

Keyword

Microsatellite instability; Ovarian neoplasm; hMLH1; hMSH2; Immunohistochemistry

MeSH Terms

Carcinogenesis
DNA Mismatch Repair
Immunohistochemistry
Incidence
Microsatellite Instability*
Microsatellite Repeats*
Mitosis
Ovarian Neoplasms
Polymerase Chain Reaction
Sensitivity and Specificity