J Korean Med Sci.  2003 Jun;18(3):387-391. 10.3346/jkms.2003.18.3.387.

Characterization of Mutator Pathway in Younger-age-onset Colorectal Adenocarcinomas

• Roh SA
• Kim HC
• Kim JS
• Kim JC

• KMID: 2157655
• DOI: http://doi.org/10.3346/jkms.2003.18.3.387

Abstract

The high-frequency microsatellite instability (MSI-H) phenotype, frequently identified in hereditary nonpolyposis colorectal cancer (HNPCC), also accounts for approximately 15% of sporadic colorectal cancers. Microsatellite instability (MSI) occurs from the mutational inactivation of the DNA mismatch repair genes, i.e. hMSH2 and hMLH1 in HNPCC, as well as from epigenetic inactivation of hMLH1 in sporadic colorectal tumors. The mutator pathway including microsatellite instability, hMLH1 promoter methylation, and hMSH2 and hMLH1 mutation patterns were identified in 21 sporadic colorectal adenocarcinoma patients younger than 30 yr excluding HNPCC. More than half of tumors showed MSI, with five MSI-H and six MSI-L (low-frequency microsatellite instability). Three of six MSI-H tumors showed the hMLH1 promoter methylation and did not express the hMLH1 protein. On the other hand, all MSI-L and all MSS (microsatellite stable) tumors expressed both hMSH2 and hMLH1 proteins. Two novel mutations, i.e. a missense mutation in hMLH1 and a splice-site alteration in hMSH2, were identified in two patients respectively. Although mutator pathway was implicated in younger-age-onset colorectal carcinogenesis, many tumors appeared to evolve from different genetic events other than hMSH2 and hMLH1 mutations frequently identified in HNPCC.

Keyword

Colorectal Neoplasms; DNA Repair; Microsatellite Repeats; Methylation

MeSH Terms

Adenocarcinoma/*genetics
Adult
Age of Onset
Colorectal Neoplasms/*genetics
DNA Methylation
DNA Repair/genetics
Female
Human
Male
Microsatellite Repeats
Mutation, Missense
Neoplasm Proteins/*genetics
Promoter Regions (Genetics)
Prospective Studies
Proteins/*genetics
Registries