Korean J Obstet Gynecol.  2003 Sep;46(9):1736-1740.

Activation of Nuclear Factor Kappa B by Inducers in Gestational Tissues at Term

• Kim JH
• Kim YA
• Yang SH

Abstract

OBJECTIVE
Nuclear factor kappa B (NF-kappa B) is a transcriptional factor in the expression of cyclooxygenase 2 (COX-2), the key enzyme in production of prostaglandins. The purpose of this study was to investigate the activation of NF-kappa B in human gestational tissues obtained from term pregnant women by various inducers.
METHODS
Myometrium, chorion, and amnion were collected during cesarean section from term pregnant women not in labor. Cells from gestational tissues were isolated and cultured with Interleukin-1beta (IL-1beta), Tumor Necrosis Factor-alpha (TNF-alpha) or Lipopolysaccharide (LPS). The activation of NF-kappa B in cells of each tissues was measured by luciferase assay.
RESULTS
Luciferase activity analysis showed significantly higher activity of NF-kappa B in myometrial cells treated with LPS, in chorion cells treated with IL-1beta and TNF-alpha, and in amnion cells treated with IL-1beta and LPS than control.
CONCLUSION
In cells of gestational tissue at term pregnancy, the activation of NF-kappa B is cell-specific and various according to inducers.

Keyword

NF-kappa B; Myometrium; Amnion; Chorion; Cytokines; Lipopolysacchride

MeSH Terms

Amnion
Animals
Cesarean Section
Chorion
Cyclooxygenase 2
Cytokines
Female
Humans
Interleukin-1beta
Luciferases
Mice
Myometrium
NF-kappa B*
Pregnancy
Pregnant Women
Prostaglandins
Tumor Necrosis Factor-alpha
Cyclooxygenase 2
Cytokines
Interleukin-1beta
Luciferases
NF-kappa B
Prostaglandins
Tumor Necrosis Factor-alpha