Korean J Obstet Gynecol.  2006 Jan;49(1):24-30.

Subcellular localization of nuclear factor kappa B in term human fetal membranes and myometrium during labor

• Choi SJ
• Moon SO
• Kim HS
• Oh SY
• Roh CR
• Kim JH

Abstract

OBJECTIVE
To investigate the subcellular localization of nuclear factor kappa B (NF-kappa B) in term human fetal membranes and myometrium during labor.
METHODS
Fetal membranes and myometrial tissue were collected from term pregnant women undergoing cesarean delivery after labor (n=4) and before labor (n=4). An immunostaining was done with NF-kappa B p65 antibody. The intensity and distribution of nuclear immunostaining of NF-kappa B p65 subunit were evaluated visually using a semiquantitative analysis.
RESULTS
NF-kappa B p65 was present in all tissues studied, and it was localized principally in the cytoplasm of cells of amnion and chorion. But, NF-kappa B p65 was localized more abundant in the nucleus than in the cytoplasm in myometrial cells. In amnion, chorion and myometrium, the staining scores of nuclear NF-kappa B did not show any difference between the after-labor group and before-labor group.
CONCLUSION
In human term gestational tissues, subcellular localization of NF-kappa B showed cytoplasmic predominance in amnion and chorion, and nuclear predominance in myometrium. But these subcellular localizations did not change during labor.

Keyword

NF-kappa B; Myometrium; Fetal membranes; Labor

MeSH Terms

Amnion
Animals
Chorion
Cytoplasm
Extraembryonic Membranes*
Female
Humans*
Mice
Myometrium*
NF-kappa B*
Pregnant Women
Transcription Factor RelA
NF-kappa B
Transcription Factor RelA