Korean J Orthod.  1979 Dec;9(1):9-14.

Studies on the protein biosynthesis in isolated periodontal ligament

• Chung HI

Abstract

The purpose of this study was to pursue the biosynthesis of proteins of human and bovine periodontal ligaments in vitro system. The excised periodontal ligaments from human and bovine were incubated in Krebs-glucose medium containing 3H-proline. After incubation the incubated periodontal ligaments were homogenized and the proteins were treated with 0.1% sodium dodecyl sulfate and beta-mercaptoethanol. Separation of the protein fractions was performed with agarose gel column chromatography and SDS acrylamide gel electrophoresis. The results indicated as follow: 1. Only a small percentage of 3H-proline incorporated into proteins was hydroxylated to 3H-hydroxyproline. 2. The labeled proteins in periodontal ligaments showed a wide distribution of molecular weight. But only small amounts of labeled protein were found that were chatacteristics of the molecular weight of collagen. 3. In all of the combined fractions of gel filtration, the degree of hydroxylation was small.

MeSH Terms

Acrylamide
Chromatography
Chromatography, Gel
Collagen
Electrophoresis
Humans
Hydroxylation
Molecular Weight
Periodontal Ligament*
Protein Biosynthesis*
Sepharose
Sodium Dodecyl Sulfate
Acrylamide
Collagen
Sepharose
Sodium Dodecyl Sulfate