Korean J Nutr.  2005 Oct;38(8):649-655.

The Effect of Caffeine on 3T3-L1 Adipocyte Differentiation : A Nutrigenomical Approach

• Kim MJ
• Kim Y
• Chung JH
• Kim JW
• Kim HK

Abstract

Nutrigenomics refers to research that investigates the interaction between nutrition and the human genome. Caffeine in tea and coffee is widely and routinely consumed by people. This study was performed to confirm the effect of caffeine treatment on the gene expression and cytokine profiling in 3T3-L1 adipocyte cells using microarray and protein array methodology. Treatment of caffeine in 3T3-L1 adipocyte cells increased expression of several genes related with obesity including adipocyte C1Q and collagen domain containing (ACDC), Adipsin (ADN), uncoupling protein 3 (UCP3), while glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which is known as lipid storage enzyme, was decreased by caffeine treatment. Furthermore, cytokines, such as interleukin-3 (IL-3), interleukin-12 (IL-12), interleukin-13 (IL-13), granulocyte colony stimulating factor (GCSF), granulocyte macrophage colony stimulating factor (GM-CSF) and vascular endothelial growth factor (VEGF), were decreased in caffeine treated 3T3-L1 adipocyte cells. These results provided interesting information about the genes related with caffeine and cytokine expression profiling in obesity.

Keyword

nutrigenomics; caffeine; 3T3-L1; obesity

MeSH Terms

Adipocytes*
Caffeine*
Coffee
Collagen
Colony-Stimulating Factors
Complement Factor D
Cytokines
Gene Expression
Genome, Human
Granulocyte-Macrophage Colony-Stimulating Factor
Granulocytes
Humans
Interleukin-12
Interleukin-13
Interleukin-3
Nutrigenomics
Obesity
Oxidoreductases
Protein Array Analysis
Tea
Vascular Endothelial Growth Factor A
Caffeine
Coffee
Collagen
Colony-Stimulating Factors
Complement Factor D
Cytokines
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-12
Interleukin-13
Interleukin-3
Oxidoreductases
Tea
Vascular Endothelial Growth Factor A