Korean J Dermatol.
2008 Nov;46(11):1470-1477.
The Production and Functions of Reactive Oxygen Species in Mouse Bone Marrow-derived Dendritic Cells by Various Haptens and Irritants
- Affiliations
-
- 1Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea. mglee@yuhs.ac
- 2Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea.
- 3Brain Korea 21 Project for Medical Science,Yonsei University College of Medicine, Seoul, Korea.
- 4Department of Dermatology, Pochon CHA University College of Medicine, Seongnam, Korea.
Abstract
- BACKGROUND
Various allergens and irritants induced the production of reactive oxygen species (ROS) in the well-established mouse dendritic cell (DC) line XS106 and this production of ROS was inhibited by antioxidants.
OBJECTIVE
To investigate the production and functions of ROS in mouse bone marrow-derived DCs (BM-DCs) by various haptens and irritants, we examined the production of ROS, the expression of surface molecules, and the production of interleukin-12 (IL-12) in mouse BM-DCs.
METHODS
Six to eight-week-old female C57/BL6 mice were used in this study. Mouse BM-DCs were co-cultured with DNFB, DNCB, TNBS, hydroquinone, NiSO4, CoCl2, MnCl2, thimerosal, SDS, and BKC. The production of ROS and the expression of surface molecules (CD40, CD80, CD86, and MHC-II) were measured by flow cytometry in chemical-treated mouse BM-DCs. In addition, the cells were pretreated with antioxidants to determine whether the production of ROS can be inhibited. The production of IL-12 was also measured in DNCB and SDS-treated mouse BM-DCs using ELISA.
Results
The production of ROS in mouse BM-DCs was induced by various allergens, including DNFB, DNCB, TNBS, hydroquinone, MnCl2 and irritants like SDS, BKC. The expression of surface molecules was induced by various chemicals and NiSO4 was the most potent inducer of surface molecules in mouse BM-DCs. The production of ROS in DNCB and SDS-treated mouse BM-DCs was partially inhibited by diphenylene iodonium, but not by rotenone, vitamin E, allopurinol, glutathione. The production of IL-12 was not detected in DNCB and SDS-treated mouse BM-DCs.
CONCLUSION
The production of ROS was induced in mouse BM-DCs by various allergens and irritants. The expression of surface molecules was also induced by various chemicals. The production of ROS was partially inhibited by DPI. The production of IL-12 was not detected.
Keyword
MeSH Terms
-
Allergens
Allopurinol
Animals
Antioxidants
Chlorides
Dendritic Cells
Dinitrochlorobenzene
Dinitrofluorobenzene
Female
Flow Cytometry
Glutathione
Haptens
Humans
Hydroquinones
Interleukin-12
Irritants
Manganese Compounds
Mice
Onium Compounds
Reactive Oxygen Species
Rotenone
Thimerosal
Vitamin E
Vitamins
Allergens
Allopurinol
Antioxidants
Chlorides
Dinitrochlorobenzene
Dinitrofluorobenzene
Glutathione
Haptens
Hydroquinones
Interleukin-12
Irritants
Manganese Compounds
Onium Compounds
Reactive Oxygen Species
Rotenone
Thimerosal
Vitamin E
Vitamins
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