Int Neurourol J.  2010 Aug;14(2):69-77.

Intravesical PGE2 Administration in Conscious Rats as an Experimental Model of Detrusor Overactivity Observed by Simultaneous Registrations of Intravesical and Intraabdominal Pressures

• Jin LH
• Han JU
• Park CS
• Shin HY
• Yoon SM
• Lee T

Abstract

PURPOSE
The urodynamic effects of intravesical PGE2 instillation on bladder function and detrusor overactivity (DO) during the filling phase were investigated in rats by measuring intraabdominal and intravesical pressures simultaneously.
MATERIALS AND METHODS
Continuous cystometry was performed inconscious, female and male Sprague- Dawley rats. We investigated pressure-, volume-, and DO-related parameters.
RESULTS
Intravesical instillation of PGE2 increased all pressure-related parameters and decreased volume-related ones, compared to the control cystometric ones. However, among the total number of intravesical pressure rises (IVPRs) above 2 cmH2O during the filling phase, only 33% in female rats and 38% in male rats after PGE2 instillation were identified as true DO during the filling phase.
CONCLUSIONS
Our findings suggest that the rat model with intravesical PGE2 is inappropriate for observing the effects of some drugs or mechanisms on DO, because only approximately 30% of IVPRs were confirmed as true DO. However, this model of intravesical PGE2 instillation has some advantages for the observation of changes in pressure and volume parameters rather than in DO-related ones.

Keyword

Rat; Detrusor overactivity; Prostaglandin E2; Pressure; Urodynamics

MeSH Terms

Administration, Intravesical
Animals
Dinoprostone
Female
Humans
Male
Models, Theoretical
Rats
Urinary Bladder
Urodynamics
Dinoprostone