Korean J Androl.  1999 Dec;17(3):143-149.

Regulation of TGF-beta 1 and Collagen Expression by Antisense Oligodeoxynucleotide in Human Corpus Cavernosum Smooth Muscle Cells

• Park K
• Kim HJ
• Lee JE
• Lee SC
• Ryu SB
• Lee HC

Abstract

PURPOSE: Transforming growth factor (TGF)-beta 1 stimulates collagen synthesis in human corpus cavernosum smooth cells (HCCSMC). We studied whether an antisense TGF-beta 1 oligodeoxynucleotide changed TGF-beta 1 mRNA expression and its related collagen synthesis.
MATERIALS AND METHODS
The HCCSMC were cultured primarily from three impotent patients. Expression of TGF-beta 1 mRNA was induced by phorbol 12-myristate 13-acetate (PMA) 0.2 micro gram/ml in the presence or absence of antisense TGF-beta 1 oligodeoxynucleotide (5'-GGGCGGCATGGGGGA-3') 20 micro gram/ml. The effect of the antisense oligodeoxynucleotide on TGF-beta 1 mRNA and collagen I synthesis were measured by RT-PCR or Northern blot analysis.
RESULTS
The expression of TGF-beta 1 mRNA in response to PMA was inhibited by treatment with the antisense oligodeoxynucleotide. Collagen I expression was also induced by PMA treatment, and the antisense oligodeoxynucleotide downregulated this expression.
CONCLUSIONS
Antisense TGF-beta 1 oligodeoxynucleotide can suppress the expression of TGF-beta 1 and TGF-beta 1-induced collagen I synthesis in HCCSMC.

Keyword

Collagen; Corpus cavernosum; Transforming growth factor beta

MeSH Terms

Blotting, Northern
Collagen*
Humans*
Muscle, Smooth*
Myocytes, Smooth Muscle*
RNA, Messenger
Transforming Growth Factor beta*
Transforming Growth Factors
Collagen
RNA, Messenger
Transforming Growth Factor beta
Transforming Growth Factors