Korean J Anat.  1997 Aug;30(4):343-350.

Chromosomal Mapping and Brain Distribution of alpha1 Subunit of N-type Voltage Dependent Calcium Channel

• Park SH
• Kim H
• Kim HR
• Park SY
• Yoo IJ
• Seo YS

Abstract

Voltage dependent calcium channels mediate wide variety of physiological functions including neurotransmitter release, neurite outgrowth, and gene expression in neurons. omega-Conotoxin-sensitive N-type calcium channels are exclusively expressed in nervous system and involved in the control of neurotransmitter release from neurons. In this experiment, I have investigated human chromosomal location and rat neuronal distribution of N-type voltage dependent calcium channel alpha1, subunit [alpha1B]. I have localized human alpha1B subunit gene to the long arm of chromosome 9[9q34] by fluorescent in situ hybridization. The distribution of rat alphaB1 subunit mRNA has been examined in the rat brain by in situ hybridization histochemistry and high level of alpha1B subunit mRNA has been observed in olfactory bulb, anterior olfactory nucleus, cerebral cortex, piriform cortex, hippocampus, dentate gyrus, parabrachial nucleus, and cerebellum and low level of expression was also found in other areas of rat brain.

Keyword

N; Fluorescent in situ hubridzation; in situ hybridzation N-type voltage dependent calcium channel; Human genom; Nervous tissue; in situ hybridization histochemistry

MeSH Terms

Animals
Arm
Brain*
Calcium Channels*
Calcium Channels, N-Type
Calcium*
Cerebellum
Cerebral Cortex
Dentate Gyrus
Gene Expression
Hippocampus
Humans
In Situ Hybridization
In Situ Hybridization, Fluorescence
Nervous System
Neurites
Neurons
Neurotransmitter Agents
Olfactory Bulb
Rats
RNA, Messenger
Calcium
Calcium Channels
Calcium Channels, N-Type
Neurotransmitter Agents
RNA, Messenger