Korean J Anat.  1999 Feb;32(1):25-33.

Localization and mRNA Expression of Angiogenin in Bovine Ovary

• Lee HS
• Lee IS
• Kang TC
• Seo J
• Song SH

Abstract

Ovary is one of the organs in which angiogenesis occurs during ovarian cycle. Angiogenesis is associated with angiogenic factor like acidic fibroblast growth factor, basic fibroblast growth factor and transformation growth factor. Therefore, we performed this study to identify the distribution and mRNA expression of angiogenin, new potential angiogenic factor, in ovary of Korean native cattle by immunohistochemistry and in situ hybridization. Angiogenin immunoreactivity and mRNA expression were observed in endothelial cells, fibroblast and vascular smooth muscle cells. However, we could not observed angiogenin immunoreactivity and mRNA expression in primordial ovarian follicle. In follicular epithelial cells of primary ovarian follicle, weak angiogenin immunoreactivity and mRNA expression were observed. Follicular epithelial cells, theca interna and externa in secondary ovarian follicles, showed angiogenin immunoreactivity, while follicular epithelial cells did the weak mRNA expression. Angiogenin immunoreactivity and mRNA expression were observed in follicular epithlial cells, theca interna and oocyte in tertiary ovarian follicle. The corpus luteum showed strong immunoreactivity and mRNA expression but atretic follicle weak. However, these angiogenin immunoreactivity and mRNA expression became to be weaker during regression. These results suggest that angiogenin may play a role as not only an angiogenic factor but a growth factor in ovary.

Keyword

angiogenin; ovary; immunohistochemistry; in situ hybridization; Korean native cattle; bovine

MeSH Terms

Angiogenesis Inducing Agents
Animals
Cattle
Corpus Luteum
Endothelial Cells
Epithelial Cells
Female
Fibroblast Growth Factor 1
Fibroblast Growth Factor 2
Fibroblasts
Immunohistochemistry
In Situ Hybridization
Menstrual Cycle
Muscle, Smooth, Vascular
Oocytes
Ovarian Follicle
Ovary*
RNA, Messenger*
Theca Cells
Angiogenesis Inducing Agents
Fibroblast Growth Factor 1
Fibroblast Growth Factor 2
RNA, Messenger