Korean J Anat.  2000 Dec;33(6):763-771.

Localization of phospholipase D genes on the human chromosome and various tissues of rat

• Roh SY
• Jeon J
• Uhm CS
• Ryu SH
• Park KW
• Suh YS
• Chun YH
• Park SH

Abstract

Phospholipase D (PLD) catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. A variety of signal molecules such as hormones, neurotransmitters, extracellular matrix molecules, and growth factors are known to induce the activation of PLD in a wide range of cell types. Hence PLD is implicated in a broad spectrum of physio-logical processes and diseases, including mitogenesis, cell differentiation, metabolic regulation, secretion, neural and cardiac stimulation, inflammation, oncogenesis, and diabetes. The signal-dependent activation of PLD has been observed in a variety of brain and neural-derived cells. In this paper, human chromosomal locations and developmental neural expression patterns in rat of PLD1 and PLD2 were investigated with fluorescent in situ hybridization (FISH) and in situ hybridization histochemistry, respectively. The PLD1 was assigned to human chromosome 3q26 and expressed most strikingly in selected ventricular neural cells lining spinal cord and brain during neuronal differentiation and migration period. The PLD2 was assigned to human chromosome 17p13.1 and expressed in differentiating ventricular neural cells and multiple regions of the postnatal rat brain.

Keyword

PLD1; PLD2; FISH; in situ hybridization histochemistry

MeSH Terms

Animals
Brain
Carcinogenesis
Cell Differentiation
Choline
Chromosomes, Human*
Extracellular Matrix
Humans
Humans*
Hydrolysis
In Situ Hybridization
In Situ Hybridization, Fluorescence
Inflammation
Intercellular Signaling Peptides and Proteins
Neurons
Neurotransmitter Agents
Phosphatidic Acids
Phosphatidylcholines
Phospholipase D*
Phospholipases*
Rats*
Spinal Cord
Choline
Intercellular Signaling Peptides and Proteins
Neurotransmitter Agents
Phosphatidic Acids
Phosphatidylcholines
Phospholipase D
Phospholipases