J Korean Ophthalmol Soc.  2002 Sep;43(9):1774-1780.

Effects of Glutathione on Reactive Oxygen Species-Induced Cytotoxicity in Human Retinal Pigment Epithelial Cell Line

• Koh JW
• Lee BR
• Ji NC

Abstract

PURPOSE: Oxidative stress is the well known causative factor for retinal damage. This study investigated the effects of glutathione on reactive oxygen species(ROS) induced injury in human retinal pigment epithelial(HRPE) cells. OBJECTS AND METHODS: HRPE cells (ATCC:CRL-2302) were cultured with DMEM media and exposed to oxidative stress (paraquat, hydrogen peroxide) and/or glutathione modulator[(buthionine sulfoximine (BSO), glutathione (GSH), 2-oxo 4-thiazolidine carboxylic acid (OTC)] for 2 days. The cell viability was determined by measuring the amount of reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT).
RESULTS
The rate of MTT reduction of HRPE cells decreased by either paraquat or hydrogen peroxide treatment. BSO as a inhibitor of glutathione biosynthesis potentiated paraquat- or hydrogen peroxideinduced HRPE cells injury. On the other hand GSH or OTC reduced the rate of decrement of MTT reduction in HRPE cells by paraquat and hydrogen peroxide.
CONCLUSIONS
Glutathione seemed to play some role in prevention of ROS-induced HRPE cells injury and OTC may be used as an agents for prevention of free radical induced HRPE cell injury.

Keyword

Glutathione; Human retinal pigment epithelial cell; Paraquat; Hydrogen peroxide

MeSH Terms

Cell Survival
Epithelial Cells*
Glutathione*
Hand
Humans*
Hydrogen
Hydrogen Peroxide
Oxidative Stress
Oxygen*
Paraquat
Retinaldehyde*
Glutathione
Hydrogen
Hydrogen Peroxide
Oxygen
Paraquat
Retinaldehyde