J Korean Ophthalmol Soc.  1999 Dec;40(12):3368-3376.

An Experimental Study of the Effect of TGF-beta1 on the Growth and Apoptosis in Y-79 Retinoblastoma Cells

• Lee MA
• Kwak HW
• Jin KH
• Kim JM

Abstract

The effect of TGF-beta1 on the growth of Y-79 retinoblastoma cells was examined. Y-79 retinoblastoma cells treated with TGF-beta1[0.5 ng/ml, 1.0ng/ml] were cultured and their growth curve was compared to the untreated cultured cells. Phase contrast microscopy, transmission electron microscopy and flow cytometer were also performed. Administration of TGF-beta1 significantly inhibited growth of Y-79 cells[p<0.05]. Transmission electron microscopic examination of those cells showed nuclear condensation, fragmentation and cytoplasmic blebbing, suggesting apoptosis as an underlying mechanism. And apoptosis was quantified by flow cytometric analysis. TGF-beta1 significantly induced apoptosis in Y-79 cells[p<0.05]. The histogram of TGF-beta1 treated Y-79 cells showed an increase in G2/M populations and decrease in G1 cell populations. This study suggested that TGF-beta1 induced apoptosis in Y-79 retinoblastoma cells, and the most plausible mechanism of growth inhibition and this function might be related to G2/M rather than G1 of the cell cycle related to Rb protein.

Keyword

Apoptosis; Retinoblastoma cells; TGF-beta1

MeSH Terms

Apoptosis*
Blister
Cell Cycle
Cells, Cultured
Cytoplasm
Microscopy, Electron, Transmission
Microscopy, Phase-Contrast
Retinoblastoma Protein
Retinoblastoma*
Transforming Growth Factor beta1*
Retinoblastoma Protein
Transforming Growth Factor beta1