Ann Dermatol.  2008 Sep;20(3):113-119. 10.5021/ad.2008.20.3.113.

Differential Expression of TGF-beta Isoforms in Human Kerationocytes by Narrow Band UVB

• Jung MC
• Shin MK
• Hong KK
• Jeong KH
• Kim NI

• KMID: 2156375
• DOI: http://doi.org/10.5021/ad.2008.20.3.113

Abstract

BACKGROUND: Transforming growth factor-beta (TGF-beta), a multifunctional growth factor, has three isoforms: TGF-beta1, TGF-beta2, and TGF-beta3. Different isoforms of TGF-beta are associated with different proliferation and differentiation states of the epidermis. Narrow band ultraviolet B (NBUVB) emits a concentrated UVB source of 311 nm. NBUVB 1,000 mJ/cm2 induces apoptosis in approximately 50% of keratinocytes.
OBJECTIVE
The purpose of this study was to evaluate whether irradiation with NBUVB would alter the expression and production of TGF-beta1, 2, and 3.
METHODS
We measured TGF-beta1, 2, and 3 mRNA and TGF-beta1 and 2 protein levels at 800, 1,000, and 1,200 mJ/cm2 for 24 hours and 48 hours.
RESULTS
TGF-beta1 mRNA levels were increased at both 24 hr and 48 hr, TGF-beta2 mRNA levels were decreased at both 24 hr and 48 hr, and TGF-beta3 mRNA levels were increased at 24 hr and similar to control at 48 hr. TGF-beta1 protein levels were increased at 48 hr but decreased at 24 hr. TGF-beta2 protein levels were decreased at both 24 hr and 48 hr.
CONCLUSION
The results suggest a possible role for TGF-beta1 after NBUVB irradiation and opposing roles for TGF-beta1 and TGF-beta2 isoforms in NBUVB irradiation.

Keyword

Keratinocytes; NBUVB; Transforming growth factor beta (TGF-beta)

MeSH Terms

Apoptosis
Enzyme Multiplied Immunoassay Technique
Epidermis
Humans
Keratinocytes
Protein Isoforms
RNA, Messenger
Transforming Growth Factor beta
Transforming Growth Factor beta1
Transforming Growth Factor beta2
Transforming Growth Factor beta3
Protein Isoforms
RNA, Messenger
Transforming Growth Factor beta
Transforming Growth Factor beta1
Transforming Growth Factor beta2
Transforming Growth Factor beta3

Figure

• 

  Fig. 1 TGF-β1 protein levels at 800, 1,000 and 1,200 mJ/cm2 for 24 hr and 48 hr when compared with the control group. TGF-β1 protein levels in supernatant was determined by ELISA assay (duplicates). Values are the mean±SD of 6 samples in 2 expriments. *p<0.05 when compared with the control group.

• 

  Fig. 2 TGF-β2 protein levels at 800, 1,000 and 1,200 mJ/cm2 for 24 hrs and 48 hrs when compared with the control group. TGF-β2 protein levels in human keratinocyte supernatant was determined by ELISA assay. Values are the mean±SD of 6 samples in 2 expriments. *p<0.05 when compared with the control group.

• 

  Fig. 3 TGF-β1 mRNA levels at 800, 1,000 and 1,200 mJ/cm2 compared with control group for 24 hr and 48 hr. (A) Representative RT-PCR showing TGF-β1 mRNA in all samples. (B) Graphical representation of combined densitometric analyses of TGF-β1 mRNA levels. Results are represented as the ratio of expression of TGF-β1 to that of β-actin (n=6). *p<0.05 when compared with control group.

• 

  Fig. 4 TGF-β2 mRNA levels at 800, 1,000 and 1,200 mJ/cm2 compared with control group for 24 hrs and 48 hrs. (A) Representative RT-PCR showing TGF-β2 mRNA in all samples. (B) Graphical representation of combined densitometric analyses of TGF-β2 mRNA levels. Results are represented as the ratio of expression of TGF-β2 to that of β-actin (n=6). *p<0.05 when compared with control group.

• 

  Fig. 5 TGF-β3 mRNA levels at 800, 1,000 and 1,200 mJ/cm2 compared with control group for 24 hr and 48 hr. (A) Representative RT-PCR showing TGF-β3 mRNA in all samples. (B) Graphical representation of combined densitometric analyses of TGF-β3 mRNA levels. Results are represented as the ratio of expression of TGF-β3 to that of β-actin. (n=6). *p<0.05 when compared with control group.

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