J Korean Soc Plast Reconstr Surg.  2010 Jul;37(4):317-322.

Difference of Gene Expression between Hypertrophic Scar Keratinocytes and Normal Keratinocytes

• Choi SW
• Chung HY
• Lim YK
• Kim HN
• Oh JW
• Kim MK
• Jeon SH
• Hong YT

Abstract

PURPOSE
There is no clear evidence of the original cause of hypertrophic scar, and the effective method of treatment is not yet established. Recently the steps of searching in gene and molecular level are proceeding. we are trying to recognize the difference between keratinocytes of hypertrophic scar and normal skin. Then we do support the comprehension of the scar formation mechanism and scar management.
METHODS
Total RNAs were extracted from cultured keratinocytes from 4 hypertrophic scars and normal skins. The cDNA chips were prepared. A total of 3063 cDNAs from human cDNA library were arrayed. And the scanning data were analyzed.
RESULTS
On microarray, heat shock protein, pyruvate kinase, tumor rejection antigen were more than 2 fold intensity genes. Among them, heat shock 70 kd protein showed the strongest intensity difference.
CONCLUSION
In this study, it can be concluded that heat shock proteins play an important role in the process of wound healing and scar formation. This study provides basic biologic information for scar research. The new way of the prevention and treatment of scar formation would be introduced with further investigations.

Keyword

Hypertrophic scar; Keratinocyte; Heat shock protein

MeSH Terms

Cicatrix
Cicatrix, Hypertrophic
Comprehension
DNA, Complementary
Gene Expression
Gene Library
Heat-Shock Proteins
Hot Temperature
Humans
Keratinocytes
Pyruvate Kinase
Rejection (Psychology)
RNA
Shock
Skin
Wound Healing
DNA, Complementary
Heat-Shock Proteins
Pyruvate Kinase
RNA