J Lung Cancer.  2012 Dec;11(2):71-76. 10.6058/jlc.2012.11.2.71.

MDA-7/IL-24 Expression and Its Relation with Clinicopathologic Factors in Lung Adenocarcinomas of 3 cm or Less in Diameter

• Roh MS
• Choi PJ
• Son C
• Lee SK

• KMID: 2199832
• DOI: http://doi.org/10.6058/jlc.2012.11.2.71

Abstract

PURPOSE
The melanoma differentiation-associated gene-7 (MDA-7) protein, also known as interleukin 24 (IL-24), is a novel candidate of tumor suppressor that has been found to experimentally induce apoptosis and growth inhibition in a variety of human malignant cells. However, there have been few studies about its role in lung adenocarcinoma. Even at the same stage and with similar pathologic characteristics, lung adenocarcinomas with a diameter of 3 cm or less can have a variable prognosis depending on their biologic characteristics. The purpose of this study is to define the relationship between MDA-7/IL-24 expression and the progression of small-sized lung adenocarcinomas.
MATERIALS AND METHODS
We performed immunohistochemical detection of MDA-7/IL-24 in forty-seven tissue samples from primary lung adenocarcinomas of < or =3 cm in diameter by using tissue microarray.
RESULTS
MDA-7/IL-24 immunoreactivity was observed in 20 (42.6%) of the 47 adenocarcinoma cases. MDA-7/IL-24 expression was positive in 66.7% of the adenocarcinomas < or =2 cm, and in 31.3% of the adenocarcinomas >2 cm or < or =3 cm in diameter. A statistically significant association was found between MDA-7/IL-24 expression and tumor size (p=0.03). Although this difference did not reach statistical significance, tumors with a negative MDA-7/IL-24 expression tended to more frequently show lymph node metastasis (p=0.07). There were no significant associations for other clinicopathologic characteristics.
CONCLUSION
These results suggest the possible involvement of MDA-7/IL-24 in the growth and progression of small-sized lung adenocarcinoma. MDA-7/IL-24 immunoreactivity could be used to identify a subset of adenocarcinomas of the lung of 3 cm or less in diameter that have different biologic behavior.

Keyword

Interleukin-24; Adenocarcinoma; Lung; Immunohistochemistry

MeSH Terms

Adenocarcinoma
Apoptosis
Humans
Immunohistochemistry
Interleukins
Lung
Lung Neoplasms
Lymph Nodes
Melanoma
Neoplasm Metastasis
Population Characteristics
Prognosis
Adenocarcinoma
Interleukins
Lung Neoplasms

Figure

• 

  Fig. 1 Immunohistochemical staining of melanoma differentiation-associated gene-7 (MDA-7)/interleukin 24 (IL-24). The expression of MDA-7/IL-24 protein was detected in the cytoplasm of both normal and tumor cells. (A) In the normal lung, the nonneoplastic alveolar epithelial cells (arrows) and macrophages were occasionally reactive for MDA-7/IL-24 (×100). (B) In the tumor tissue, MDA-7/IL-24 immunoreactivity was observed in 20 (42.6%) of the 47 adenocarcinoma cases (×200).

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