J Korean Orthop Res Soc.  2014 Jun;17(1):1-12. 10.0000/jkors.2014.17.1.1.

The Effects of Substrates and Hydrostatic Pressure on Differentiation of Mesenchymal Stem Cell

• An KC
• Park DH
• Choi JS
• Kong GM
• Kim JY
• Ha BH
• Shin JW
• Park SH
• Kang YG

• KMID: 2199037
• DOI: http://doi.org/10.0000/jkors.2014.17.1.1

Abstract

PURPOSE
This study investigated the potential of dual differentiation of stem cells into osteo- and chodrogenesis depending on scaffold type even in the same environment.
MATERIALS AND METHODS
For the part of the cartilage tissue section, MSCs were suspended in alginate solution and bead droplets were made using 23G syringe. For the bone tissue section, PCL/HA scaffolds were made using the bio-plotting system followed by seeding mesenchymal stem cells (MSCs) onto the scaffolds. Scaffolds with MSCs were cultured in cocktail media containing osteogenic and chondrogenic growth factors for up to 21 days. To provide mechanical environments which articular cartilage experiences in-vivo, intermittent hydrostatic pressure (IHP) was engaged. Various cellular responses were assessed: the quantitative analysis of DNA contents, GAG contents, ALP activities and immunofluorescence.
RESULTS
We found that IHP promoted MSCs differentiation into the targeted cell types. That is, MSCs in alginate scaffolds were able to be differentiated into chondrocytes, while those onto PCL/HA scaffolds were able to be differentiated into osteoblasts.
CONCLUSION
Depending on the scaffold characteristics MSCs can be differentiated into bone cells or chondrocytes. This technique can provide a cue for the treatment of osteochondral defects utilizing tissue engineering.

Keyword

Osteochondral defects; Poly (epsilon-caprolactone); Alginate; Mesenchymal stem cells; Intermittent hydrostatic pressure (IHP)

MeSH Terms

Bone and Bones
Cartilage
Cartilage, Articular
Chondrocytes
Cues
DNA
Fluorescent Antibody Technique
Hydrostatic Pressure*
Intercellular Signaling Peptides and Proteins
Mesenchymal Stromal Cells*
Osteoblasts
Stem Cells
Syringes
Tissue Engineering
DNA
Intercellular Signaling Peptides and Proteins