J Korean Orthop Res Soc.  2012 Jun;15(1):1-10.

Fabrication of a Scaffold for Bone Regeneration and Differentiation of Mesenchymal Stem Cells

• An KC
• Shin JW
• Kim YJ
• Jung DW
• Park DH
• Kim DM

Abstract

PURPOSE
This in-vitro study aimed to confirm the clinical potential of a newly suggested bone substitute fabricated by a bio-plotting system.
MATERIALS AND METHODS
A 3-dimensional, PCL (polycaprolactone)-based bone substitute was fabricated by a bio-plotting system, which enables us to control the shape and porosity. Mesenchymal stem cells(MSCs) from a New Zealand white rabbits were seeded to the bone substitute. Through 2-week experiment, morphological and various biological responses were assessed depending on the use of osteogenic media and/or mechanical stimuli.
RESULTS
The viability of cells was confirmed. Other findings in relation to proliferation and differentiation were also confirmed to be biocompatible. Specifically, the activity of ALP was increased in the groups using osteogenic media along the time. And the expressions collagen type I was more affected by intermittent hydrostatic pressure rather than by osteogenic media. But those of Cbfa-1 was affected by osteogenic media and mechanical stimuli, as well.
CONCLUSION
The potential of a newly fabricated and suggested material was confirmed. However, further long term and animal study are recommanded for the clinical application.

Keyword

Mesenchymal stem cells; Intermittent hydrostatic pressure; Microenvironment; Physical stimuli; Osteogenesis; Bone regeneration

MeSH Terms

Animals
Bone Regeneration
Bone Substitutes
Collagen Type I
Durapatite
Hydrostatic Pressure
Mesenchymal Stromal Cells
Osteogenesis
Porosity
Rabbits
Seeds
Bone Substitutes
Collagen Type I
Durapatite