J Korean Neurosurg Soc.  2014 Mar;55(3):125-130. 10.3340/jkns.2014.55.3.125.

The Neuroprotective Effects of Carnosine in Early Stage of Focal Ischemia Rodent Model

Affiliations
  • 1Department of Neurosurgery, Seoul Medical Center, Seoul, Korea.
  • 2Research Institute, Seoul Medical Center, Seoul, Korea. dohheekim@gmail.com

Abstract


OBJECTIVE
This study was conducted to elucidate neuroprotective effect of carnosine in early stage of stroke.
METHODS
Early stage of rodent stroke model and neuroblastoma chemical hypoxia model was established by middle cerebral artery occlusion and antimycin A. Neuroprotective effect of carnosine was investigated with 100, 250, and 500 mg of carnosine treatment. And antioxidant expression was analyzed by enzyme linked immunosorbent assay (ELISA) and western blot in brain and blood.
RESULTS
Intraperitoneal injection of 500 mg carnosine induced significant decrease of infarct volume and expansion of penumbra (p<0.05). The expression of superoxide dismutase (SOD) showed significant increase than in saline group in blood and brain (p<0.05). In the analysis of chemical hypoxia, carnosine induced increase of neuronal cell viability and decrease of reactive oxygen species (ROS) production.
CONCLUSION
Carnosine has neuroprotective property which was related to antioxidant capacity in early stage of stroke. And, the oxidative stress should be considered one of major factor in early ischemic stroke.

Keyword

Focal ischemia; Carnosine; Early stage; Neuroprotective effect

MeSH Terms

Anoxia
Antimycin A
Blotting, Western
Brain
Carnosine*
Cell Survival
Enzyme-Linked Immunosorbent Assay
Infarction, Middle Cerebral Artery
Injections, Intraperitoneal
Ischemia*
Neuroblastoma
Neurons
Neuroprotective Agents*
Oxidative Stress
Reactive Oxygen Species
Rodentia*
Stroke
Superoxide Dismutase
Antimycin A
Carnosine
Neuroprotective Agents
Reactive Oxygen Species
Superoxide Dismutase
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