Chonnam Med J.  2011 Dec;47(3):144-149. 10.4068/cmj.2011.47.3.144.

Time Point Expression of Apoptosis Regulatory Proteins in a Photochemically-Induced Focal Cerebral Ischemic Rat Brain

• Kim HS
• Park MS
• Lee JK
• Kim HJ
• Park JT
• Lee MC

• KMID: 2172222
• DOI: http://doi.org/10.4068/cmj.2011.47.3.144

Abstract

Apoptosis after global or focal cerebral ischemia plays a crucial role in mediating cell death. In this study, we observed the time point expression of physiologic events involving apoptosis regulatory proteins after photochemically-induced focal cerebral ischemia in Sprague-Dawley rats. Protein expression was evaluated at days 1, 3, and 7 by Western blot. Bcl-2, Bax, caspase-3, and phosphorylated Akt (pAkt) activity markedly increased in the ischemic hemisphere in a time-dependent manner, not affected. The expression of Bcl-2, Bax, and caspase-3 was dramatically changed around day 3, whereas changes in pAkt expression occurred at day 1. Differential elevation of these apoptosis regulatory proteins at various time points indicates that different modes of cell death occur in photochemically-induced focal cerebral ischemia in a rat brain.

Keyword

Brain ischemia; Apoptosis; Rose bengal

MeSH Terms

Animals
Apoptosis
Apoptosis Regulatory Proteins
Blotting, Western
Brain
Brain Ischemia
Caspase 3
Cell Death
Negotiating
Rats
Rats, Sprague-Dawley
Rose Bengal
Apoptosis Regulatory Proteins
Caspase 3
Rose Bengal

Figure

• 

  FIG. 1 Representative Western blots showing immunoreactivity of Bcl-2, Bax, caspase-3, pAkt, and survivin proteins in controls and in the ischemic hemisphere 1, 3, and 7 days after photochemically induced focal cerebral ischemia. All proteins were detected at the predicted molecular size and showed different time point expression patterns. The results of GAPDH analysis are shown as an internal control.

• 

  FIG. 2 Relative changes in apoptosis-associated protein expression. Data are expressed as the mean±SD expression ratio of the ischemic hemisphere to sham controls (n=5, each group). *p<0.05; **p<0.01.

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