J Bacteriol Virol.  2013 Jun;43(2):99-110. 10.4167/jbv.2013.43.2.99.

Characterization of Community-Associated Methicillin-Resistant Staphylococcus aureus in Gwangju, Korea

• Kee HY
• Kim MJ
• Kim SH
• Lee SM
• Kim SK
• Ha DR
• Kim ES
• Chung JK

• KMID: 2168679
• DOI: http://doi.org/10.4167/jbv.2013.43.2.99

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important nosocomial pathogens worldwide. This study was performed to investigate the characterization of MRSA isolated from healthy persons in Gwangju area. A total of 404 nasal swab samples was collected during October 2011 and May 2012 in Gwangu, Korea. A survey on MRSA was conducted with meat distributors (n=230), pre-school children (n=108), officers (n=66), respectively. To confirm the MRSA, polymerase chain reaction (PCR) for the S. aureus specific gene and mecA gene was performed. A total of 34 (8.4%) MRSA isolates was isolated from 404 nasal swab samples: 6.1% (14/230) from meat distributors, 16.7% (18/108) from pre-school children, and 3.0% (2/66) from officers samples, respectively. The most prevalent antimicrobial resistance observed in the MRSA isolates was to ampicillin 100% (34/34), followed by penicillin 97.1% (33/34), oxacillin 94.1% (32/34) and erythromycin 52.9% (18/34). All MRSA isolates were then characterized by panton-valentine leukocidin (pvl) gene detected by PCR, staphylococcal cassette chromosome mec (SCCmec) typing, and pulsed-field gel electrophoresis (PFGE) with Sma I digestion. 34 MRSA isolates from nasal carriage were pvl gene negative, SCCmec type IV; 73.5% (25/34), type II; 17.6% (6/34), type III; 2.9% (1/34), and untypable; 5.9% (2/34), respectively. 34 MRSA isolates showed 16 PFGE patterns. These results indicated that isolation rates of community-associated methicillin-resistant S. aureus (CA-MRSA) from healthy persons were low (8.4%), but continuous surveillance and monitoring should be performed to prevent the spread of MRSA in the community.

Keyword

Community-associated methicillin-resistant S. aureus; mecA gene; Staphylococcal cassette chromosome mec type; Pulsed-field gel electrophoresis

MeSH Terms

Adenosine
Ampicillin
Bacterial Toxins
Child
Digestion
Electrophoresis, Gel, Pulsed-Field
Erythromycin
Exotoxins
Humans
Korea
Leukocidins
Meat
Methicillin Resistance
Methicillin-Resistant Staphylococcus aureus
Oxacillin
Penicillins
Polymerase Chain Reaction
Adenosine
Ampicillin
Bacterial Toxins
Erythromycin
Exotoxins
Leukocidins
Oxacillin
Penicillins

Figure

• 

  Figure 1. Antimicrobial resistance patterns of community-associated methicillin-resistant S. aureus (CA-MRSA) isolates. S, susceptible; I, intermediate; R, resistant; AM ampicillin; FEP cefepime; CTT cefotetan; CIP ciprofloxacinem; C chloramphenicol; CC clindamycin; E erythromycin; CN amikacin; IPM imipenem; OX oxacillin; P penicillin; RA rifampin; TE tetracycline; SXT trimethoprim/sulfamethoxazole; VA vancomycin; FOX cefoxitin; CRO ceftriaxone; TEL telithromycin; AmC amoxicillin/clavulanic; LZD linenzolid; KF, cephalothin

• 

  Figure 2. Dendrogram of Sma I-digested pulsed-field gel electrophoresis (PFGE) profiles of community-associated methicillin-resistant S. aureus (CA-MRSA) isolates.

• 

  Figure 3. Pulsed-field gel electrophoresis (PFGE) Sma I-pattern of community-associated methicillin-resistant S. aureus (CA-MRSA) isolates.

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