Immune Netw.  2006 Mar;6(1):27-32. 10.4110/in.2006.6.1.27.

Regulation of Inflammatory Repertoires and NF-kappaB Signal Transduction by DDB, an Active Compound from Schizandra Chinensis Baillon

• Joo SS
• Yoo YM
• Won TJ
• Kim MJ
• Lee SG
• Hwang KW
• Lee DI

• KMID: 2167986
• DOI: http://doi.org/10.4110/in.2006.6.1.27

Abstract

BACKGROUND: Chronic inflammation in the brain has known to be associated with the development of a various neurological diseases including dementia. In general, the characteristic of neuro-inflammation is the activated microglia over the brain where the pathogenesis occurs. Pro-inflammatory repertoires, interleukin-1beta (IL-1beta) and nitric oxide (NO), are the main causes of neuro-degenerative disease, particularly in Alzheimer's disease (AD) which is caused by neuronal destruction. Those pro-inflammatory repertoires may lead the brain to chronic inflammatory status, and thus we hypothesized that chronic inflammation would be inhibited when pro-inflammatory repertoires are to be well controlled by inactivating the signal transduction associated with inflammation.
METHODS
In the present study, we examined whether biphenyl dimethyl dicarboxylate (DDB), an active compound from Schizandra chinensis Baillon, inhibits the NO production by a direct method using Griess reagent and by RT-PCR in the gene expression of inducible nitric oxide synthase ((i)NOS) and IL-1beta. Western blots were also used for the analysis of NF-kappaB and IkappaB.
RESULTS
In the study, we found that DDB effectively inhibited IL-1beta as well as NO production in BV-2 microglial cell, and the translocation of NF-kappaB was comparably inhibited in the presence of DDB comparing those to the positive control, lipopolysaccharide.
CONCLUSION
The data suggested that the DDB from Schizandra chinensis Baillon may play an effective role in inhibiting the pro-inflammatory repertoires which may cause neurodegeneration and the results imply that the compound suppresses a cue signal of the microglial activation which can induce the brain pathogenesis such as Alzheimer's disease.

Keyword

Nitric oxide; iNOS; NF-KB; IL-1beta; biphenyl; dimethyl dicarboxylate; alzheimer's disease

MeSH Terms

Alzheimer Disease
Blotting, Western
Brain
Cues
Dementia
Gene Expression
Inflammation
Interleukin-1beta
Microglia
Neurons
NF-kappa B*
Nitric Oxide
Nitric Oxide Synthase Type II
Schisandra*
Signal Transduction*
Interleukin-1beta
NF-kappa B
Nitric Oxide
Nitric Oxide Synthase Type II