Int J Stem Cells.  2012 Nov;5(2):125-129.

SIRT1 Inhibits p53 but not NF-kappaB Transcriptional Activity during Differentiation of Mouse Embryonic Stem Cells into Embryoid Bodies

• Lhee SJ
• Song EK
• Kim YR
• Han MK

Abstract

BACKGROUND AND OBJECTIVES
SIRT1, a histone diacetylase, modify transactivation function of various transcription factor including p53 and NF-kappaB. p53 and NF-kappaB is involved in in vitro differentiation of mouse embryonic stem cells (mESC) into mouse embryoid body (mEB). These suggest that SIRT1 might affect in vitro differentiation of mESC into mEB by regulation of p53 and NF-kappaB.
METHODS AND RESULTS
In this study we analyzed the effect of SIRT1 in in vitro differentiation of mESC into mEB using wild and SIRT1 knockout mESC. To examine SIRT1-specific gene in mESC, this study conducted microarray-based differential gene expression analysis between wild and SIRT1 knockout mESC. Comparing their gene expression patterns, this study determined a list of genes regulated by SIRT1. cDNA microarray data-set analysis revealed that genes associated with transcription and signal transduction are significantly modified in SIRT1 knockout mESC. cDNA microarray data-set analysis between mESC and EB in wild and SIRT1 showed that SIRT1 inhibits p53 signaling pathway but not affect NF-kappaB signaling pathway.
CONCLUSIONS
This study suggests that SIRT1 modify mESC differentiation by regulation of p53 transcriptional activity.

Keyword

SIRT1; Embryonic stem cells; p53; NF-kappaB; Embryoid bodies

MeSH Terms

Animals
Embryoid Bodies
Embryonic Stem Cells
Gene Expression
Histones
Mice
NF-kappa B
Oligonucleotide Array Sequence Analysis
Signal Transduction
Transcription Factors
Transcriptional Activation
Histones
NF-kappa B
Transcription Factors