Gut Liver.
2010 Mar;4(1):84-93.
Membrane Proteome Analysis of Cerulein-Stimulated Pancreatic Acinar Cells: Implication for Early Event of Acute Pancreatitis
- Affiliations
-
- 1Department of Biotechnology, Brain Korea 21 Project, The Catholic University of Korea, Bucheon, Korea.
- 2Department of Pharmacology, Yonsei University College of Medicine, Seoul, Korea.
- 3Department of Food and Nutrition, Brain Korea 21 Project, Yonsei University College of Human Ecology, Seoul, Korea. kim626@yonsei.ac.kr
Abstract
- BACKGROUND/AIMS
Cerulein pancreatitis is similar to human edematous pancreatitis with dysregulation of the production and secretion of digestive enzymes, edema formation, cytoplasmic vacuolization and the death of acinar cells. We hypothesized that membrane proteins may be altered as the early event during the induction of acute pancreatitis. Present study aims to determine the differentially expressed proteins in the membranes of cerulein-treated pancreatic acinar cells.
METHODS
Pancreatic acinar AR42J cells were treated with 10(-8) M cerulein for 1 hour. Membrane proteins were isolated from the cells and separated by two-dimensional electrophoresis using pH gradients of 5-8. Membrane proteins were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis of the peptide digests. The differentially expressed proteins, whose expression levels were more or less than three-fold in cerulein-treated cells, were analyzed.
RESULTS
Two differentially expressed proteins (mannan-binding lectin-associated serine protease-2, heat shock protein 60) were up-regulated while four proteins (protein disulfide isomerase, gamma-actin, isocitrate dehydrogenase 3, seven in absentia homolog 1A) were down-regulated by cerulein treatment in pancreatic acinar cells. These proteins are related to cell signaling, oxidative stress, and cytoskeleton arrangement.
CONCLUSIONS
Oxidative stress may induce cerulein-induced cell injury and disturbances in defense mechanism in pancreatic acinar cells.
MeSH Terms
-
Acinar Cells
Actins
Caerulein
Cytoplasm
Cytoskeleton
Edema
Electrophoresis
Heat-Shock Proteins
Humans
Isocitrate Dehydrogenase
Isocitrates
Mass Spectrometry
Membrane Proteins
Membranes
Oxidative Stress
Pancreatitis
Protein Disulfide-Isomerases
Proteins
Proteome
Proton-Motive Force
Serine
Actins
Caerulein
Heat-Shock Proteins
Isocitrate Dehydrogenase
Isocitrates
Membrane Proteins
Protein Disulfide-Isomerases
Proteins
Proteome
Serine
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