Allergy Asthma Immunol Res.  2015 Jul;7(4):346-358. 10.4168/aair.2015.7.4.346.

Distinct Inflammatory Profiles in Atopic and Nonatopic Patients With Chronic Rhinosinustis Accompanied by Nasal Polyps in Western China

Affiliations
  • 1Department of Otolaryngology, Head and Neck Surgery, West China Hospital, Sichuan University, Chengdu, China.
  • 2Department of Otolaryngology, People's Hospital of the Tibet Autonomous Region, Lasha, China.
  • 3Department of Geriatrics, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China. bhanor@163.com
  • 4Department of Otolaryngology, Head and Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China. allergyli@163.com

Abstract

PURPOSE
The role of systemic sensitization in the pathophysiology of chronic rhinosinusitis with nasal polyps (CRSwNP) remains elusive. This study sought to characterize the pattern of cytokines in polyp tissues from atopic and nonatopic patients with CRSwNP.
METHODS
Atopic and nonatopic polyp and normal tissues were collected from 70 CRSwNP patients and 26 control subjects, respectively. The distribution of inflammatory cells (eosinophils, neutrophils, mast cells, etc.) were examined using immunohistochemistry, the mRNA levels of the transcription factors GATA-3, T-bet, RORc, and FOXP3 were determined using quantitative real-time polymerase chain reaction. The levels of inflammatory mediators (IFN-gamma, IL-5, IL-17A, etc.) in tissue homogenates were measured using enzyme-linked immunosorbent assay (ELISA). Moreover, the levels of inflammatory mediators in the supernatant of anti-IgE stimulated polyp tissues were measured using ELISA.
RESULTS
Atopic CRSwNP patients were characterized by increased eosinophil accumulation, enhanced eosinophilic inflammation (elevated IL-5, ECP, and total IgE), and significantly increased GATA-3 mRNA levels (P<0.05), whereas both atopic and non-atopic CRSwNP patients showed decreased FOXP3 mRNA expression (P<0.05). After addition of anti-IgE stimulation, atopic CRSwNP patients produced more IL-5, IL-2, IL-10, IL-17A, and PGD2 in the supernatant of stimulated polyp tissues than nonatopic CRSwNP patients did.
CONCLUSIONS
Atopic and nonatopic CRSwNP patients may possess the patterns of inflammatory response in polyp tissues.

Keyword

Nasal polyps; chronic rhinosinusitis; atopy; cytokine; eosinophil; IgE

MeSH Terms

China*
Cytokines
Enzyme-Linked Immunosorbent Assay
Eosinophils
Humans
Immunoglobulin E
Immunohistochemistry
Inflammation
Interleukin-10
Interleukin-17
Interleukin-2
Interleukin-5
Mast Cells
Nasal Polyps*
Neutrophils
Polyps
Prostaglandin D2
Real-Time Polymerase Chain Reaction
RNA, Messenger
Transcription Factors
Cytokines
Immunoglobulin E
Interleukin-10
Interleukin-17
Interleukin-2
Interleukin-5
Prostaglandin D2
RNA, Messenger
Transcription Factors
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